冰茶栓对星形胶质细胞增殖、凋亡及细胞周期的影响

目的:应用血清药理学观察冰茶栓(BCS)对体外培养星形胶质细胞(Astrocyte AST)增殖、凋亡及细胞周期的影响。方法:原代培养SD乳鼠大脑皮层AST,将纯化后的细胞经免疫细胞化学鉴定后,随机分为药物组(高、中、低剂量)和对照组,加入相应血清共培养48h后,MTT比色法观察AST的增殖情况,流式细胞仪检测AST的凋亡率和细胞周期的变化。结果:MTT结果显示,冰茶栓高、中剂量组较空白血清组细胞数量明显减少(P<0.01,P<0.05),低剂量组与空白血清组比较无显著差异;流式细胞仪检测结果显示,冰茶栓组细胞凋亡比例较对照组明显增加(P<0.01);冰茶栓组G1期细胞比例较对照组明显提高(P<0.01),S期和G2/M期细胞比例较对照组明显降低(P<0.01)。结论:冰茶栓对体外培养的星形胶质细胞增殖具有明显的抑制作用,并可诱导其凋亡,抑制其由G1期向S期和G2/M期的转化。

Effects of Bingcha Embolus on the proliferation,apoptosis and cell cycle of astrocytes

AIM: To study the effects of Bingcha Embolus on the proliferation, apoptosis and cell cycle of astrocytes in vitro in terms of serum pharmacology. METHODS: The cerebral cortex astrocytes of neonatal Sprague-Dawley rats were cultivated in vitro, and the purified astrocytes were randomly devided into Bingcha Embolus groups (high dose, middle dose, low dose) and control group after being identified by means of immunocytochemical method. After the incubation of 48 h with sera, MTT was employed as a proper method to detect the proliferation of astrocytes. The ratio of apoptosis and different cell cycle phases were measured by Flow Cytometry. RESULTS: The results of MTT showed that the cell number of Bingcha Embolus groups (high dose, middle dose) were obviously fewer than that of the control group (P<0.01, P<0.05), but there was no much difference between the low dose group and control group; the results of Flow Cytometry showed that the ratio of apoptosis in Bingcha Embolus groups was higher than that of control group (P<0.01), the ratio of cells on G_1 phase was higher in Bingcha Embolus groups than that in the control group (P<0.01), and the ratios of cells on S phase and G_2/M phase were lower in Bingcha Embolus groups than that in the control group (P<0.01). CONCLUSION: Bingcha Embolus obviously inhibits the proliferation of astrocytes incubated in vitro, induces their apoptosis, and prevents the cell transformation from G_1 phase to S phase and G_2/M phase.