| 血清胰岛素TRFIA的建立 |
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| 引用本文： | 姚焱,李国祥,陈键. 血清胰岛素TRFIA的建立[J]. 标记免疫分析与临床, 2012, 19(1): 36-39 |
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| 作者姓名： | 姚焱 李国祥 陈键 |
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| 作者单位： | 原子高科股份有限公司,北京,102413;中国同位素有限公司,北京,100045 |
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| 摘 要： | 目的 建立血清胰岛素( Ins)时间分辨荧光免疫分析法(time-resolved fluoroimmunoassay,TRFIA).方法 采用一株Ins-MAb包被于固相微孔板上,另一株Ins-MAb用DTTA- Eu3+标记作为示踪剂采用Victor 1420荧光仪测定,.并对本法进行评价.结果 本法的标准曲线范围...
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| 关 键 词： | 胰岛素 铕 时间分辨荧光免疫分析 单克隆抗体 血清 |
Establishment of TRFIA for Serum Insulin |
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| YAO Yan , LI Guo-xiang , CHEN Jian. Establishment of TRFIA for Serum Insulin[J]. Labeled Immunoassays and Clinical Medicine, 2012, 19(1): 36-39 |
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| Authors: | YAO Yan LI Guo-xiang CHEN Jian |
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| Affiliation: | (Atom High Tech Co., Ltd, Beijing 102413, China) |
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| Abstract: | Objective A time-resolved fluoroimmunoassay （TRFIA） for the measurement of insulin （Ins） in human serum had been established. Methods The test was based on the one-step sandwich technique with two MAbs directed against two different epitopes of Ins using coated 96-well mierotitre plate as the solid phase and DITA-Eu3＋ as the label, and the established method was evaluated with Victor 1420 muhilabel counter. Results The standard ranges were 5 - 160mU/L. The detection limit was 0.35mU/L. At three different levels of Ins, the intra-assay CV was 0.90% to 4.10%, the inter-assay CV was 4.55% to 4.96%. The average re- covery rates were 97.7% - 102.9%. The detected results with this method closely corresponded to those of by ELISA with DSL （R = 0. 982, n = 80）. Conclusion The established TRFIA in this study was specific, sensi- tive, easy to use, and suitable for clinical serum insulin measurement. |
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| Keywords: | Insulin Europium Time-resolved fluoroimmunoassay Monoantibody Serum |
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