富血小板血浆中血小板浓度对血小板聚集的影响

目的探讨富血小板血浆（PRP）血小板浓度对血小板聚集的影响。方法随机收集107名门诊体检健康志愿者静脉血浆,分离PRP和PPP（贫血小板血浆）后,用血液分析仪测定PRP血小板浓度,按PRP浓度用自身PPP配成50×109/L～400×109/L浓度梯度的血浆,用血浆比浊法测血小板聚集率。结果 PRP血小板浓度在50×109/L～400×109/L,二磷酸腺苷（ADP）和花生四烯酸（AA）诱导的血小板聚集率分别为（10.4±8.7）%至（55.3±9.9）%和0%至（75.2±10.1）%,血小板聚集率随血小板浓度的增高而增高,血小板浓度为250×109/L时,聚集率增幅减缓,血小板浓度小于150×109/L时,血小板聚集率明显降低。结论 PRP血小板浓度对血小板聚集的影响明显,血小板聚集试验PRP血小板浓度大于150×109/L;血浆比浊法血小板聚集试验适宜的血小板浓度为250×109/L。

Effect of Platelet Concentration of Platelet-rich Plasma on Platelet Aggregation

Objective To explore the effect of platelet concentration of platelet-rich plasma（PRP） on platelet aggregation.Methods 107 blood samples of outpatient volunteers were randomly collected and centrifuged,PRP and PPP（platelet-poor plasma）were gotten,then platelet concentration of PRP was detected by the blood analyzer and diluted with their own PPP into a series of platelet concentration of plasma in the gradient： 50×109/L～ 400×109/L,then platelet aggregation rates of the plasma were detected according to the standard protocols by plasma turbidimetry.Results The platelet concentrations of the plasma were in the range of 50×109/L～400×109/L,and the platelet aggregation rate gradually increased with a higher concentration of platelet,but slowly at the concentration of 250×109/L.When platelet concentrations 150×109/L,the platelet aggregation rate decreased markedly.The platelet aggregation rates induced by ADP and AA were（10.4±8.7）%～（55.3±9.9）% and 0%～（75.2±10.1）%,respectively.Conclusion The effect of platelet concentration of PRP on platelet aggregation was significantly.The platelet concentration of PRP should be 150×109/L at least for routine platelet aggregation tests.The platelet concentration of 250×109/L seems to be the optimal concentration for platelet aggregation by plasma turbidimetry.