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Detection of Plasmodium vivax infection in the Republic of Korea by loop-mediated isothermal amplification (LAMP) |
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| Authors: | Jun-Hu Chen Feng Lu Chae Seung Lim Heui-June Ahn Satoru Takeo Jetsumon Sattabongkot |
| Institution: | a Department of Parasitology, Kangwon National University College of Medicine, Hyoja2-dong, Chunchon, Gangwon-do 200-701, Republic of Korea b Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou 310013, People's Republic of China c Jiangsu Institute of Parasitic Diseases, Wuxi 214064, People's Republic of China d Department of Laboratory Medicine, College of Medicine, Korea University, Seoul 425-707, Republic of Korea e Department of Malaria and Parasitic Disease, National Institute of Health, KCDC, Seoul 122-701, Republic of Korea f Department of Internal Medicine, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, Republic of Korea g Department of Laboratory Medicine, Kangwon National University College of Medicine, Chunchon 200-701, Republic of Korea h Cell-free Science and Technology Research Center and Venture Business Laboratory, Ehime University, Matsuyama 790-8577, Japan i Department of Entomology, Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand |
| Abstract: | Loop-mediated isothermal amplification (LAMP) is a novel technique that rapidly amplifies target DNA in isothermal conditions. In a previous study, the sensitivities and specificities of LAMP, microscopy, and nested PCR were compared in the context of rapid malaria detection. In the present study, LAMP detected vivax malaria parasites in 115 of 117 microscopically positive samples (sensitivity, 98.3%; 95% CI, 97.4-100%), which agreed well with the nested PCR results (sensitivity, 99.1%; 95% CI: 96.0-100%). No positive cases of malaria were detected by LAMP or nested PCR in 50 consecutive feverish patients other than malaria from malaria endemic areas. LAMP performed on DNA extracted from heat-treated blood had a sensitivity of 93.3% (28/30, 95% CI: 84.4-100%) and specificity of 100% (30/30, 95% CI: 100%). The present study shows that LAMP based assays have high sensitivity, specificity, and amplification efficiencies for Plasmodium vivax detection. The authors recommend that LAMP can be considered as a rapid nucleic acid amplification assay for the molecular diagnosis of P. vivax in both clinical laboratories and malaria clinics in areas where vivax malaria is endemic. |
| Keywords: | Malaria Plasmodium vivax LAMP Diagnosis Republic of Korea |
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