Effects of vitamin D and retinoic acid on human glioblastoma cell lines |
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Authors: | L Magrassi G Butti S Pezzotta L Infuso G Milanesi |
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Institution: | (1) Department of Surgery, Section of Neurosurgery, University of Pavia, IRCCS Policlinico S. Matteo, Pavia, Italy;(2) IGBE CNR, Pavia, Italy |
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Abstract: | Summary The biological significance of vitamin D receptors expressed by glioblastoma and other glial tumours is still unclear. In an effort to clarify this issue we studied the effects of increasing concentrations of 25-dihydroxyvitamin D3 and its metabolite 1 , 25-dihydroxyvitamin D3 on two human glioblastoma cell lines. Both substances were capable of inducing a significant (> 50%) reduction in growth of the two glioblastoma cell lines at dosages over 5 M. When the HU 70 cell line was treated by increasing dilutions of 25-dihydroxyvitamin D3 combined with 1 M all trans-retinoic acid, significant inhibition was apparent even after addition of 25-dihydroxyvitamin D3 in the nanomolar range. Reduction of growth index was mainly due to induced cell death.Our results providein vitro evidence that vitamin D metabolites alone or in combination with retinoids may be potentially useful agents in the differentiation therapy of human malignant gliomas. |
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Keywords: | Glioblastoma retinoic acid vitamin D cell death |
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