Infection of cynomolgus macaques with a recombinant monkeypox virus encoding green fluorescent protein |
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| Authors: | Goff Arthur Mucker Eric Raymond Jolynne Fisher Robert Bray Mike Hensley Lisa Paragas Jason |
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| Affiliation: | (1) Virology Division, Viral Therapeutics Branch, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter St., Fort Detrick, MD 21702-5011, USA;(2) Armed Forces Institute of Pathology, Silver Spring, MD, USA;(3) Laboratory of Plasma Derivatives, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA;(4) Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, MD 21702, USA |
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| Abstract: | Monkeypox virus (MPXV) causes a vesiculopustular rash illness resembling smallpox in humans and produces a similar disease in nonhuman primates. To enhance the ability of researchers to study experimental MPXV infections, we inserted a gene encoding green fluorescent protein (GFP) into Monkeypox virus Zaire-79. Wild-type and MPXV-GFP replicated with similar kinetics in cell culture and caused a similar disease when injected intravenously into cynomolgus macaques. In MPXV-GFP-infected animals, examination under fluorescent light facilitated the identification of skin lesions during disease development and internal sites of replication at necropsy. MPXV-GFP could improve the quantitative assessment of antiviral therapy and vaccine efficacy. |
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