输血四项检测中化学发光微粒免疫分析技术的价值及应用

［摘要］ 目的探讨化学发光微粒免疫分析技术（chemiluminescent microparticle immunoassay，CMIA）在检测乙型肝炎表面抗原（hepatitis B surface antigen，HBsAg）、人类免疫缺陷病毒（human immunodeficiency virus，HIV）、丙型肝炎病毒（hepatitis C virus，HCV）、梅毒螺旋体（treponema pallidum，TP）抗体输血四项指标中的价值。 方法选择进行输血前四项检查的患者300例。采用CMIA检测患者的血清标本，与病理检查结果比较。 结果CMIA检测血清HBsAg、HIV抗体、TP抗体、HCV抗体的结果与临床病理检测结果比较，差异无统计学意义（χ2=0.000、0.000、0.267、0.150，P＞0.05）。CMIA检测HBsAg、HIV抗体敏感度、特异度、阳性预测值、阴性预测值、诊断符合率皆为100.00%；CMIA检测TP抗体敏感度、特异度、阳性预测值、阴性预测值、诊断符合率分别为93.33%、95.00%、94.91%、93.44%、94.17%；CMIA检测HCV抗体敏感度、特异度、阳性预测值、阴性预测值及诊断符合率分别为95.00%、91.67%、91.93%、94.83%、93.33%。 结论在输血四项指标检测中，CMIA与病理诊断结果比较，虽然存在一定的差异，但是表现出良好的敏感度和特异度，具有较高的阳性预测值、阴性预测值及诊断符合率。

The value and application of chemiluminescence microparticle immunoassay in four blood transfusion assays

［Abstract］ObjectiveTo investigate the chemiluminescent microparticle immunoassay(CMIA) in the detection of hepatitis B surface antigen(HBsAg), human immunodeficiency virus(HIV), hepatitis C virus(HCV), the value and application of four tests for treponema pallidum(TP) antibody transfusion.〖WTHZ〗MethodsA retrospective analysis of 300 patients was performed in our hospital who performed the four tests before blood transfusion. CMIA was used to detect serum samples from patients, and then compared with clinical diagnosis results. 〖WTHZ〗ResultsThe results of CMIA detection of serum HBsAg, HIV antibody, TP antibody and HCV antibody were not significantly different from those of clinical pathological findings(χ2=0.000, 0.000, 0.267, 0.150, P＞0.05). The CMIA for detecting HBsAg, HIV antibody sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic coincidence rate were all 100%; CMIA for detecting TP antibody sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic compliance The rates were 93.33%, 95.00%, 94.91%, 93.44%, and 94.17%, respectively. The sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic coincidence rate of HCV antibody detected by CMIA were 95.00%, 91.67%, and 91.93%, 94.83%, 93.33%, respectively. 〖WTHZ〗ConclusionIn the detection of four indicators of blood transfusion, CMIA and the pathological diagnosis results, although there are certain differences, showed good sensitivity and specificity, with high positive predictive value, negative predictive value and diagnostic coincidence rate.