组织工程重建胰腺脱细胞支架血管系统的研究*

目的：使用内皮祖细胞再内皮化胰腺脱细胞支架，并初步评价其在宿主体内生成血管的能力。方法：采用灌注法制备结构完整的全胰腺脱细胞支架；并提取大鼠骨髓单个核细胞，诱导分化为内皮祖细胞，进行原代培养；将培养完成的内皮种植于支架内，并行体内移植。结果：在移植后第10天，实验组中包含红细胞的血管管腔数为（43±6）cm2，而对照组为（8±3）cm2；在移植后第20天，实验组中包含红细胞的血管管腔数为（62±9）cm2，而对照组为（16±5）cm2，此结果具有统计学意义（P<0.05，n=6）。种植内皮祖细胞后，支架在体内能更快更多的生成新生血管，与对照组比较差异有统计学意义（P<0.05）。结论：再血管化的支架在体内移植后，与对照组相比，可以更快的与宿主循环系统形成吻合。

Vascularization of Pancreatic De-Cellularized Scaffold for Tissue Engineering

Objective: To recellularize the Pancreatic De-Cellularized Scaffold with endothelial progenitor cells (EPCs) and evaluate its potential function of endothelialization in vivo. Methods: Decellularized pancreatic scaffolds were obtained by perfusing method. Bone marrow mononuclear cells(BMMNCs) were isolated, cultured and induced into EPCs. The scaffolds were implanted in vivo after recellularization by EPCs. Results: Quantitative results show that the density of new blood vessels in the endothelialized scaffold was significantly higher than that in the control group. Conclusion: Compared with the control group, the endothelialized scaffold can be connected with the host circulatory system faster.