含Hp特异性抗体羊奶2种检测方法的比较及初步临床效果

［摘要］目的比较含抗Hp特异性抗体羊奶的2种检测方法，并初步观察抗体羊奶的临床效果。方法用国际标准菌株ATCC26695及ATCC26695、SS1混合菌液制备2种含抗Hp特异性抗体的羊奶，分别用双相免疫扩散试验及酶联免疫吸附测定(enzyme linked immunosorbent assay,ELISA) 法检测羊奶中抗Hp特异性抗体含量，并比较二者检测效果；30名受试者按照随机抽样法分为试验组和对照组，试验组每天饮用抗体羊奶，对照组饮用普通羊奶，连续饮用30 d，用14C尿素呼气试验(14C urea breath test,14C-UBT) 评估效果。结果2种方法检测结果均证实了抗体羊奶中抗Hp特异性抗体的存在，ELISA结果显示，2种抗体羊奶中抗Hp特异性抗体含量高于对照孔，差异有统计学意义(P＜0.05)，且随着稀释倍数增大，抗体含量逐渐降低；2种抗体羊奶间抗体含量差异无统计学意义(P＞0.05)。初步临床试验证明试验组经服用抗体羊奶后有效率为66.6%，与服用前比较差异有统计学意义(P＜0.05)。结论2种检测方法均可较好地反映抗体羊奶中抗Hp特异性抗体存在，ELISA法简便、快速，能够更为精确地定量检测抗体含量，所获得的结果更为可靠。临床试验表明抗体羊奶能够有效降低患者14C-UBT数值，具有良好的临床应用前景。

Comparison of the two methods in the detection of goat-milk containing anti-Helicobacter pylori specific antibody and preliminary clinical effect

［Abstract］ Objective〖HTSS〗To compare two detection methods of goat-milk containing anti-Helicobacter pylori specific antibody, and to observe the clinical effect of antibody goat-milk. Methods〖HTSS〗Goat-milk containing antibodies against Helicobacter pylori was prepared by immunizing dairy goat with international standard strains (ATCC26695, ATCC26695 and SS1 mixture). The valence of antibody against Helicobacter pylori in goat-milk was detected by double immunodiffusion test and enzyme linked immunosorbent assay(ELISA), and then compare the two methods. 30 subjects were randomly assigned to two groups. The test group were treated with goat-milk containing Helicobacter pylori specific antibody, and control group with common goat-milk, drink for 30 days continuously. The effect was assessed by the 14C urea breath test(14C-UBT). Results〖HTSS〗The results of the two methods all confirmed the existence of anti-Helicobacter pylori specific antibodies in goat milk, ELISA test show that the content of antibodies in the two goat-milk was higher than that in the control, the difference was statistically significant(P＜0.05), and with the increase of dilution ratio, the antibody content decreased gradually. There was no significant difference in antibody content between the two kinds of goat milk(P＞0.05). Preliminary clinical trials have shown that the effective rate of the experimental group was 66.6% after taking the antibody goat-milk, and the difference was statistically significant(P＜0.05) compared with the control group. Conclusion〖HTSS〗Both of the two detection methods can better prove the existence of anti-Helicobacter pylori specific antibodies in goat-milk. Methed of ELISA is simple and fast, which can more accurate quantitatively detect antibody content. And the results are more reliable. Clinical trials have shown that goat-milk containing Helicobacter pylori specific antibody can effectively reduce the value of 14C-UBT in patients, it has a good prospect of clinical application.