应用microCT技术对中药干预去卵巢小鼠骨组织微结构的分析

目的应用micro CT技术观察健骨颗粒对C57小鼠去卵巢骨质疏松模型骨组织骨量、骨微结构及生物力学的影响。方法将4周龄清洁级C57小鼠30只随机分为三组(假手术组10只、卵巢切除小鼠两组各10只),两组卵巢切除小鼠术后1周开始,分别用健骨颗粒和生理盐水进行灌胃,假手术组用生理盐水灌胃。2个月后,小鼠左胫骨行micro CT检测及图像分析、右胫骨行生物力学三点抗压最大载荷检测。结果与假手术组比较,去卵巢组BMC、BMD、Mean、BV、BS显著下降(P0.01),TMC下降(0.01P0.05);与去卵巢组比较,去卵巢中药组BMC、BMD、Mean指标显著提高(P0.01);图像分析结果显示去卵巢组较假手术组骨皮质薄,骨小梁数量少,形态细小、不连续呈扭曲或断裂状等明显骨质疏松病理特征,去卵巢中药组介于两者之间。生物力学结果显示三组胫骨三点抗压最大载荷均具有明显差异(P0.01)。结论去卵巢2月成功建立小鼠绝经后骨质疏松症模型;健骨颗粒抗骨质疏松作用明显,主要通过增加骨量和改善骨小梁微结构来最终提高骨强度;micro CT对骨质疏松参数分析简洁、高效,图像多维、全面,与传统的检测方法相比,具有一定的优势。

Analysis of the effect of traditional Chinese medicine on microstructure of the bone in ovariectomized mice using micro CT

Objective To observe the effect of the Jiangu particles on bone mass, bone micro-structure, and biomechanics in ovariectomized C57 mice using micro CT analysis. Methods Thirty 4-week-old clean grade C57 mice were randomly divided into 3 groups (10 in sham group, and 10 in each of two ovariectomized groups). One week after ovariectomy, mice in two ovariectomized groups were fed with Jiangu particles or saline, respectively. Mice in sham group were fed with normal saline. Two months later, the left tibia was detected using micro CT analysis. The right tibia was detected with 3-point maximal loading. Results Compared to those in sham group, BMC, BMD, Mean, BV, and BS in ovariectomized groups decreased significantly (P<0,01). TMC decreased (0,01 < P< 0. 05). Compared to those in the ovariectomized group, BMC, BMD, and Mean in ovariectomized with Chinese medicine group increased significantly (P <0.01). Image analysis showed that compared to sham group, thin cortical bone in OVX group had a small number of trabecular bone, tiny and discrete distorted fracture shape, or other obvious pathology of osteoporosis. The biomechanical results showed that tibia 3-point maximum load pressure between any two groups was significantly different (P <0. 01). Conclusion Postmenopausal osteoporosis model is successfully established after 2- month ovariectomy. Jiangu particles increase trabecular bone volume and improve the micro-structure and ultimately improve bone strength. Micro CT analysis is simple, efficient, multi-dimensional, and comprehensive in osteoporosis analysis. It has certain advantage comparing to traditional detection methods.