miR-141-3p表达与卵巢癌细胞顺铂化疗敏感性的相关性研究

［摘要］ 目的 探讨miR-141-3p在卵巢癌细胞对顺铂耐药中的表达和作用。 方法 采用实时荧光定量PCR法检测A2780/DDP细胞中miR-141-3p的表达水平；下调/过表达miR-141-3p后，采用CCK-8法检测卵巢癌细胞对顺铂的增殖率并计算药物IC50；采用Western blot法检测增殖相关蛋白（proliferating cell nuclear antigen,PCNA）表达水平。 结果 miR-141-3p在顺铂耐药的卵巢癌细胞株A2780/DDP中的表达显著高于顺铂敏感的卵巢癌细胞株A2780（P＜0.05）。与对照组比较，A2780细胞中过表达miR-141-3p后可显著增加细胞对顺铂的IC50，增殖相关蛋白PCNA表达亦增加（P＜0.05）。与对照组比较，A2780/DDP细胞中下调miR-141-3p表达后，细胞对顺铂的IC50显著下降，增殖相关蛋白PCNA表达下降（P＜0.05）。 结论 miR-141-3p在顺铂耐药卵巢癌细胞的耐药机制中表达增加，下调miR-141-3p可增加顺铂耐药的卵巢癌细胞对顺铂的敏感性。

The correlation between the expression of miR-141-3p and Cisplatin chemosensitivity of ovarian cancer cells

［Abstract］ Objective To investigate the expression and role of miR-141-3p in Cisplatin resistance of ovarian cancer cells. Methods RT-qPCR was performed to identify the expression of miR-141-3p in A2780/DDP cells. CCK8 assay was used to exam cell proliferation. Western blot analysis was used to detect proliferating cell nuclear antigen（PCNA） protein level. Results miR-141-3p was upregulated in DDP-resistance A2780/DDP cells compared with DDP-sensitive A2780 cells（P＜0.05）. Overexpression of miR-141-5p in A2780 cells increased the IC50 of DDP compared with negative control, and also increased PCNA protein expression（P＜0.05）. Knockdown of miR-14-5p in A2780/DDP cells decreased the IC50 of DDP compared with negative control, and also decreased PCNA protein expression（P＜0.05）. Conclusion The expression of miR-141-3p increased in DDP-resistance of ovarian cancer. Silencing of miR-141-3p can increase the chemo-sensitivity in ovarian cancer cells to DDP.