miR-655-3p在头颈鳞状细胞癌中的表达改变及其临床意义

［摘要］目的 初步探讨miR-655-3p在头颈鳞状细胞癌中的表达情况及其可能的临床意义。方法 通过实时荧光定量反转录聚合酶链反应（quantitative real-time polymerase chain reaction，qRT-PCR）的方法，检测头颈鳞状细胞癌组织及其配对癌旁正常组织中miR-655-3p的相对表达水平，并分析其相对表达水平与头颈鳞状细胞癌患者临床病理参数之间的关系。结果 miR-655-3p在头颈鳞状细胞癌组织样本中相对表达水平明显低于配对癌旁正常组织样本中（P＜0.05）；miR-655-3p高表达组和低表达组在临床分期（P=0.038）、组织分化程度（P=0.036）及是否发生颈部淋巴结转移（P=0.042）各临床特征中的构成比差异有统计学意义。而在患者性别、年龄、吸烟、饮酒等临床特征组的构成比中差异无统计学意义（P＞0.05）。结论 头颈鳞状细胞癌中miR-655-3p表达降低，其表达水平与患者临床分期、癌组织分化程度及是否伴有颈部淋巴结转移等临床特征相关，提示其可能作为抑癌基因，在头颈鳞状细胞癌中表达失活，某种程度上促进了头颈鳞状细胞癌的发生发展。

Expression and clinical significance of miR-655-3p in head and neck squamous cell carcinoma

［Abstract］ Objective〖HTSS〗To explore the expression level of miR-655-3p in head and neck squamous cell carcinoma and its potential clinical significance. 〖WTHZ〗Methods〖HTSS〗The expression levels of miR-655-3p in head and neck squamous cell carcinoma and its matched normal tissues were measured by the method of quantitative real-time polymerase chain reaction(qRT-PCR), and the correlation between the expression levels of miR-655-3p and the clinical parameters of head and neck squamous cell carcinoma were analyzed. 〖WTHZ〗Results〖HTSS〗The relative expression level of miR-655-3p in cancerous tissues of head and neck squamous cell carcinoma was significantly lower than that in matched normal tissues(P＜0.05). Clinical stage(P=0.038), differentiation degree(P=0.036) and lymph node metastasis(P=0.042) were significantly different between miR-655-3p high expression group and low expression group. However, there was no significant difference of constituent ratios in gender, age, smoking and drinking between two groups(P＞0.05). 〖WTHZ〗Conclusion〖HTSS〗The expression level of miR-655-3p in head and neck squamous cell carcinoma is lower and is related to clinical stages, differentiation degrees and lymph node metastasis, suggesting that miR-655-3p may be used as a tumor suppressor gene, and its expression is inactivated in head and neck squamous cell carcinoma, so as to promote the occurrence and development of head and neck squamous cell carcinoma to some extent.