一种研究choke 血管新的动物模型-大鼠背部皮窗

目的　建立大鼠背部皮窗来观察大鼠皮肤血管体之间的choke血管。 方法 首先设计一种可以骑跨在大鼠背部的观察窗，之后在SD大鼠背部一侧设计切取一个以髂腰动脉皮支为蒂的跨3个血管体区的皮瓣，后原位缝合。将观察窗对准皮瓣的choke区安装上去，每隔12 h在体视显微镜下观察choke血管的形态，用数码相机拍照, 连续观察7 d。 结果 10只中有3只术后出现严重感染。通过皮窗观察孔能够完整流器地观察到choke区的动静脉。皮瓣掀起60 h可见连接小静脉管径发生轻微扩张，随后几天管径继续扩张，并因为血流动力学的改变，血管的曲度增加；这些小静脉的管径扩张于术后5 d达到极致。术后choke区静脉干及动脉管径也可见相应扩张，但其变化不及静脉明显。 结论 与之前报道过的X线造影、CTA相比，大鼠背部皮窗作为观察choke血管的一种新的方法，其最大的优点是能在同一活体上对choke血管在较长时间内进行实时观察。其缺点为频繁麻醉可能影响大鼠的生理状态。

A new method for observation of choke vessels:the dorsal skinfold chamber on the rat

Objective To adopt of the dorsal skinfold chamber on the rat for observation of choke vessels between the angiosomes of the rat's skin.Methods Firstly,a dorsal skinfold chamber that could ride on the back of the rat after its install was designed;Then,a three-territory flap based on the cutaneous perforator of the ilicolumbar artery was harvested,which was sutured back in situ immediately;Finally,the dorsal skinfold chamber was installed for real-time intravital microscopic monitoring at an interval of 12h ...