| 白藜芦醇对D-半乳糖诱导的亚急性衰老小鼠海马神经元的保护作用 |
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| 引用本文： | 姚新梅,晏芳,田雪梅.白藜芦醇对D-半乳糖诱导的亚急性衰老小鼠海马神经元的保护作用[J].中国临床解剖学杂志,2011,29(6). |
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| 作者姓名： | 姚新梅 晏芳 田雪梅 |
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| 作者单位： | 1. 南方医科大学基础医学院组织学与胚胎学教研室,广州,510515
2. 南方医科大学基础医学院组织学与胚胎学教研室,广州510515;华南师范大学生命科学学院,广州510631 |
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| 基金项目： | 广东省科技计划项目(73009) |
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| 摘 要： | 目的 探讨白藜芦醇对亚急性衰老小鼠海马神经细胞的保护作用.方法 D-半乳糖颈背部皮下注射制备亚急性衰老小鼠模型,设正常对照组、对照组、高低剂量处理组和阳性对照组.第3周始,处理组分别给予白藜芦醇15、45mg/kg(低、高剂量)灌胃,对照组给予生理盐水或DMSO溶液灌胃.8周后,检测小鼠血清、脑组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量.制作石蜡切片,尼氏染色观察小鼠海马CA1区形态结构,免疫组化检测P53蛋白表达,TUNEL法检测细胞凋亡.结果 各组小鼠海马组织形态结构无明显变化；与对照组相比,白藜芦醇45 mg/kg体重组小鼠血清和脑组织中SOD活性明显升高,MDA含量明显下降；白藜芦醇45 mg/kg体重组小鼠海马CA1区P53蛋白表达阳性细胞较对照组小鼠减少,但各组均无明显的凋亡细胞.结论 白藜芦醇能增强亚急性衰老小鼠的抗氧化功能,降低亚急性衰老小鼠海马CA1区神经元P53蛋白的表达.
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| 关 键 词： | 白藜芦醇 D-半乳糖 衰老 海马神经细胞 |
Protective effects of resveratrol on hippocampus neurons of subacute aging mice |
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| YAO Xin-mei
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YAN Fang
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TIAN Xue-mei.Protective effects of resveratrol on hippocampus neurons of subacute aging mice[J].Chinese Journal of Clinical Anatomy,2011,29(6). |
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| Authors: | YAO Xin-mei YAN Fang TIAN Xue-mei |
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| Institution: | YAO Xin-mei 1,YAN Fang1,TIAN Xue-mei1,2 1.Department of Histology and Embryology,Southern Medical University,Guangzhou 510515,China,2.College of Life Science,South China Normal University,Guangzhou 510631 |
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| Abstract: | Objective To investigate the protective effect of resveratrol(RES) on neurons in hippocampus in subacute aging mouse.Methods The subacute aging mice were prepared by subcutaneous injection of D-galactose at the nape.From the third week,the mice were fed with resveratrol(15.45 mg / kg) by oral gavage and the control group were fed with normal saline or solution of DMSO.The superoxide dismutase(SOD) activity and malondialdehyde(MDA) level in serum and brain of mice were detected.Morphology of hippocampal CA1 ... |
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| Keywords: | Resveratrol D-galactose Aging Neurons in hippocampus |
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