| 精子发生障碍患者Y染色体微缺失的检测 |
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| 引用本文： | 孔琳,潘伯臣,杜强,杨大磊.精子发生障碍患者Y染色体微缺失的检测[J].中国医科大学学报,2010,39(1):37. |
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| 作者姓名： | 孔琳 潘伯臣 杜强 杨大磊 |
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| 作者单位： | 中国医科大学,附属盛京医院妇产科生殖中心,沈阳,110004 |
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| 摘 要： | 目的探讨Y染色体微缺失与精子发生障碍患者染色体核型、临床表型、性激素水平等因素的关系。方法采用多重PCR技术检测42例非梗阻性无精子症及39例严重少精子症患者的SRY基因、ZFY基因及AZFa、AZFb和AZFc3个区域的6个序列标签位点。结果81例非梗阻性无精子症及严重少精子症患者中发现了10例微缺失,发生率12.35%,其中非梗阻性无精子症患者6例,严重少精子症患者4例;10例微缺失患者中,1例为AZFa+AZFb+AZFc区缺失,2例为AZFb+AZFc区缺失,1例为AZFb区缺失,6例为AZFc区缺失。结论Y染色体长臂微缺失与精子发生障碍相关,有必要对非梗阻性无精子症及严重少精子症患者进行Y染色体微缺失检测。
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| 关 键 词： | 男性不育 无精子因子 Y染色体微缺失 多重聚合酶链反应 无精子症 严重少精子症 |
Detection of Y Chromosomal Microdeletions in Patients with Spermatogenic Failure |
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| KONG Lin
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PAN Bo-chen
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DU Qiang
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YANG Da-lei.Detection of Y Chromosomal Microdeletions in Patients with Spermatogenic Failure[J].Journal of China Medical University,2010,39(1):37. |
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| Authors: | KONG Lin PAN Bo-chen DU Qiang YANG Da-lei |
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| Institution: | KONG Lin,PAN Bo-chen,DU Qiang,YANG Da-lei (Department of Obstetrics , Gynecology,Shengjing Hospital,China Medical University,Shenyang 110004,China) |
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| Abstract: | Objective To explore the relationship between Y chromosomal microdeletions and chromosome karyotype,clinical phenotypes and sex hormone levels in patients with spermatogenic failure. Methods SRY gene,ZFY gene and 6 sequence-tagged-sites in AZFa,AZFb and AZFc were detected with multiplex polymerase chain reaction in 42 non-obstructive azoospermic and 39 severe oligozoospermic men. Results Ten cases(12.35%)of microdeletions were found in 81 non-obstructive azoospermic(6 cases)or severe oligozoospermic patient... |
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| Keywords: | male infertility azoospermia factor Y chromosomal microdeletions multiplex PCR azoospermia severe oligozoospermi |
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