| Lewis肺癌细胞通过TLR9对CD4~+CD25~+Treg细胞影响的研究 |
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| 引用本文： | 李欣,付海英,张佳伦,李一. Lewis肺癌细胞通过TLR9对CD4~+CD25~+Treg细胞影响的研究[J]. 中国免疫学杂志, 2010, 26(2) |
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| 作者姓名： | 李欣 付海英 张佳伦 李一 |
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| 作者单位： | 吉林大学白求恩医学院免疫学教研室,长春,130021 |
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| 摘 要： | 目的:本研究以Lewis肺癌细胞为研究对象,探讨肿瘤细胞通过TLRs对CD4+CD25+Treg细胞的影响。方法:我们采用流式细胞术检测了Lewis肺癌细胞与脾淋巴细胞共培养系统中CD4+CD25+Treg细胞数量变化;通过RT-PCR方法检测了共培养对Foxp3和TLR1-9mRNA表达的影响;采用TLR9受体阻断剂氯喹阻断Lewis肺癌TLR9的表达。结果:与对照组相比,共培养组CD4+CD25+Treg细胞数量及Foxp3 mRNA表达均明显增高(P0.05);Lewis肺癌细胞与淋巴细胞共培养后可影响多种TLRs表达,其中TLR9 mRNA表达与对照组相比明显增高(P0.05),阻断Lewis肺癌细胞TLR9可明显降低CD4+CD25+Treg细胞数量及Foxp3 mRNA表达(P0.05)。结论:Lewis肺癌细胞可通过TLR9促进CD4+CD25+Treg细胞产生及功能增强,参与诱导肿瘤的免疫耐受,从而促进肿瘤的发生和发展。
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| 关 键 词： | CD4~+ CD25~+ Treg细胞 肿瘤免疫 |
Lewis lung cancer cells affect CD4~+CD25~+Treg cells via TLR9 |
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| LI Xin,FU Hai-Ying,ZHANG Jia-Lun,LI Yi. Lewis lung cancer cells affect CD4~+CD25~+Treg cells via TLR9[J]. Chinese Journal of Immunology, 2010, 26(2) |
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| Authors: | LI Xin FU Hai-Ying ZHANG Jia-Lun LI Yi |
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| Affiliation: | LI Xin,FU Hai-Ying,ZHANG Jia-Lun,LI Yi.Department of Immunology,Bethune Medical College,Jilin University,Changchun 130021,China |
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| Abstract: | Objective:To explore the influence of tumor cells on CD4+CD25+Treg cells via TLRs.Methods:The numbers changes of CD4+CD25+Treg cells in the co-culture system of Lewis lung cancer cells and splenic lymphocytes were detected by flow cytometry;The expression of Foxp3 and TLR1-9 mRNA after co-culture were detected by RT-PCR;TLR9 expression on Lewis lung cancer cells was blocked by TLR9 receptor antagonist chloroquine.Results:Compared with control group,the number of CD4+CD25+Treg cells and Foxp3 mRNA expression... |
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| Keywords: | Foxp3 TLRs CD4~+ CD25~+ Treg cells Foxp3 TLRs Tumor immunity |
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