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| 中国特发性无精子症和少精子症患者雄激素受体基因CAG重复多态性研究 | |
| 引用本文： | 李座祥,唐文豪,汪朝晖,王琳,王媛媛,马潞林,马旭.中国特发性无精子症和少精子症患者雄激素受体基因CAG重复多态性研究[J].中华男科学杂志,2005,11(5):335-338,342. |
| 作者姓名： | 李座祥 唐文豪 汪朝晖 王琳 王媛媛 马潞林 马旭 |
| 作者单位： | 1. 中国协和医科大学,北京,100730;国家人口计生委科学技术研究所遗传室,北京,100081 2. 北京大学第三医院泌尿外科,北京,100083 3. 国家人口计生委科学技术研究所遗传室,北京,100081 |
| 基金项目： | 国家重点基础研究发展计划(973计划)，"中国生殖健康生物信息库的建立和应用研究"项目 |
| 摘 要： | 目的分析中国特发性无精子症和少精子症患者雄激素受体(AR)基因(CAG)n微卫星多态性并探讨该多态性与精子生成障碍发生的关系。方法应用PCR和变性聚丙烯酰胺凝胶电泳分析技术对52例少精子症患者和31例无精子症患者的外周血标本进行CAG重复数测定,分析该微卫星多态性和精子生成障碍发生的关系。结果少精子症患者组和无精子症患者组CAG重复数均数分别为22.19和22.13,CAG重复数≥28的百分率分别为1.9%和3.2%,比例逐渐升高。结论AR基因(CAG)n微卫星的CAG重复数在中国男性不育患者中呈现多态性,与精子生成障碍发生的关系有待进一步研究。 |
| 关 键 词： | 男性不育 雄激素受体基因 CAG重复序列 基因多态性 |
| 文章编号： | 1009-3591(2005)05-0335-05 |
(CAG)n Polymorphism of Androgen Receptor Gene in Idiopathic Azoospermic and Oligospermic Chinese Men |
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| LI Zuo-xiang,TANG Wen-hao,WANG Zhao-hui,WANG Lin,WANG Yuan-yuan,MA Lu-lin,MA Xu.(CAG)n Polymorphism of Androgen Receptor Gene in Idiopathic Azoospermic and Oligospermic Chinese Men[J].National Journal of Andrology,2005,11(5):335-338,342. | |
| Authors: | LI Zuo-xiang TANG Wen-hao WANG Zhao-hui WANG Lin WANG Yuan-yuan MA Lu-lin MA Xu |
| Institution: | Peking Union Medical College, Beijing 100730, China. |
| Abstract: | Objective: To research (CAG)n polymorphism of androgen receptor gene in idiopathic azoospermic and oligospermic Chinese men, and to explore its correlation with the development of dyszoospermia. Methods: Fifty-two men with oligospermia and 31 with azoospermia were enrolled, the number of CAG sequence repeats determined by PCR and denaturing polyacrylamide gel electrophoresis (PAGE), and (CAG)n polymorphism analyzed. Results: The CAG repeats in the men with oligospermia and those with azoospermia were ~22.19 and ~22.13 respectively. Conclusion: The polymorphism of the repeat length of the (CAG)n satellite in androgen receptor gene may affect spermatogenesis and have a less important role in male infertility. |
| Keywords: | male infertility androgen receptor gene (CAG)n sequence repeat genetic polymorphism |
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