自体骨髓干细胞移植治疗失代偿期肝硬化

目的探讨自体骨髓干细胞（BMSC）移植对肝硬化失代偿期患者的治疗作用。方法23例肝硬化失代偿期患者无菌抽取骨髓60～100ml，分离纯化BMSC，然后经肝动脉导管注入肝脏。术前术后定期检测肝功能，并观察同期的症状体征和不良反应情况。结果与术前比较，术后1周内所有血清学指标均无明显改变；术后1个月，白蛋白为（31．44±2．08）g／LVS（27．63±2．04）g／L（P〈0．01）；谷丙转氨酶为（58．70±25．87）U／Lvs（67．13±27．78）U／L（P〈0．01）；总胆红素、血小板等指标均无明显变化（P〉0．05）。移植后大多数患者症状改善明显，移植后1个月内腹水减轻12例（52％），食欲改善10例（43％），体力好转11例（47．8％），腹胀减轻9例（39．1％），睡眠改善12例（52．1％）。结论自体骨髓干细胞肝内移植治疗失代偿期肝硬化是一种安全的治疗措施，对失代偿期的肝脏可起到一定支持作用。

Autologous bone marrow stem cell intrahepatic transplantation for treatment of decompeusated liver cirrhosis

Objective To evaluate the effect of autologous bone marrow-derived stem cell (BMSCs) trans-plantation in the treatment of decomponsated hepatic cirrhosis. Methods Bone marrow was harvested (60～100 ml) from 23 patients in the transplantation group. The BMSCs were isolated and infused into liver of patients via he-patic artery. At different time points after the transplantation,the patients'liver function and the quantity of blood platelet (PLT)were evaluated. Results All the serum biochemical indexes remained stable 1 week after the trans-plantation. After 4 weeks, albumin level increased significantly in comparison with the preoperative level(P<0.01) and ALT was significantly lowered (P<0.01), but total hilirubin and the quantity of PLT remained unchanged(P> 0.05). 4 weeks after transplantation, ascites decreased in 12 cases(52%), appetite improved in 10 cases(43%) and abdomen distention ameliorated in 9 cases (39.1%). No adverse events developed in 23 patients with bone marrow stem cells transplantation. Conclusion BMSCs transplantation can improve the liver function of patients with decompensated liver cirrhosis and showes good safety and effectiveness.