水通道蛋白-4对蛛网膜下隙出血后脑水肿的影响

目的研究大鼠蛛网膜下隙出血(SAH)后脑水肿病理过程中水通道蛋白-4(AQP-4)的表达与脑水肿形成之间的关系。方法采用大鼠断尾取血,枕大池注入自体血的方法制作大鼠SAH模型,用干湿质量法和免疫组化法分别检测SAH后不同时间段脑含水量和AQP-4蛋白表达的变化。结果SAH后8 h,脑组织AQP-4蛋白在脑水肿区表达开始增强,至2 d达到高峰,此时脑组织严重水肿;第7天,AQP-4的表达已减少,脑水肿减轻。结论SAH后AQP-4表达明显增强,与脑水肿呈正相关关系。AQP-4可能在SAH后脑水肿的形成过程中起重要作用。

THE EFFECT OF AQUAPORIN-4 ON BRAIN EDEMA FOLLOWING SUBARACHNOID HEMORRHAGE IN RATS

Objective To study the relationship between the expression of aquaporin-4(AQP-4) and cerebral edema formation during the pathologic process following subarachnoid hemorrhage(SAH) in rats. Methods The SAH model was established by injecting autologous blood into the cisterna magna.The changes of brain water content were measured by the wet and dry weight methods.Immunohistochemistry was used to determine the changes of the expression of aquaporin-4 in cerebral cortex and thalamus on different times. Results The expression of AQP-4 in the edematous tissue increased at 8 hours following SAH.At 48 h,the expression of AQP-4 reached its maximum,and the brain edema became remarkable.On 7th day,the expression of AQP-4 in SAH group was still higher than that in the control group and the brain edema was unremarkable. Conclusion The expression of AQP-4 increases remarkably after SAH and shows positive correlation with cerebral edema,which suggests that AQP-4 play an important role in process in cerebral-edema formation following SAH.