|
|||||
|
|
| 葫芦素B体外对乳腺癌细胞的生长抑制作用研究 | |
| 引用本文： | 张美侠,杨姣,王艳丽,周雪莹,李岭,邓意辉.葫芦素B体外对乳腺癌细胞的生长抑制作用研究[J].实用药物与临床,2010,13(1):7-9. |
| 作者姓名： | 张美侠 杨姣 王艳丽 周雪莹 李岭 邓意辉 |
| 作者单位： | 1. 中国医科大学药学院临床药理教研室,沈阳,110001 2. 中国医科大学药学院遗传学教研室,沈阳,110001 3. 沈阳药科大学药剂教研室,沈阳,110015 |
| 基金项目： | 辽宁省自然科学基金资助项目(20082092);;留学回国人员科研启动基金(教外司[2006]331号) |
| 摘 要： | 目的观察葫芦素B(Cucurbitacin B)对非磷酸化STAT3人乳腺癌细胞系MCF-7和磷酸化STAT3人乳腺癌细胞系MDA-MB-435S的增殖抑制和诱导凋亡作用,为临床应用提供依据。方法用不同浓度的葫芦素B分别处理MCF-7和MDA-MB-435S细胞,48 h后用MTT法检测细胞增殖;用荧光显微镜观察细胞凋亡。结果MTT结果显示,葫芦素B对MCF-7和MDA-MB-435S细胞的增殖抑制作用具有明显的剂量依赖性,统计学分析提示,各实验组与对照组之间差异有统计学意义(P<0.05);荧光显微镜显示典型的细胞凋亡。结论葫芦素B对人乳腺癌细胞的增殖具有抑制作用(与STAT3的活化状态无关),诱导乳腺癌细胞凋亡,从而发挥抗肿瘤效应。 |
| 关 键 词： | 葫芦素B 乳腺癌 MCF-7 MDA-MB-435S 凋亡 |
Inhibitory effect of cucurbitacin B on the growth of breast cancer cells |
|
| ZHANG Mei-xia,YANG Jiao,WANG Yan-li,ZHOU Xue-ying,LI Ling,DENG Yi-hui.Inhibitory effect of cucurbitacin B on the growth of breast cancer cells[J].Practical Pharmacy and Clinical Remedies,2010,13(1):7-9. | |
| Authors: | ZHANG Mei-xia YANG Jiao WANG Yan-li ZHOU Xue-ying LI Ling DENG Yi-hui |
| Institution: | 1.a.Department of Clinical Pharmacology/a>;b.Department of Medical Genetics/a>;China Medical University/a>;Shenyang 110001/a>;China/a>;2.Department of Pharmacy/a>;Shenyang Pharmaceutical University/a>;Shenyang 110015/a>;China |
| Abstract: | Objective To investigate the inhibitory effect of cucurbitacin B on different human breast cancer cells.Methods In vitro anti-proliferative activity of cucurbitacin B was investigated on MCF-7 and MDA-MB-435S cell lines by MTT assay.Morphologic changes were evaluated under light microscope.Apoptotic cells were evaluated with Hoechst 33258 staining.Results Cucurbitacin B inhibited both STAT3 positive and STAT3 negative breast cancer cells proliferation in a dose dependent manner.Marked morphological changes ... |
| Keywords: | Cucurbitacin B Breast cancer MCF-7 MDA-MB-435S Apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |