DNA profile of dog feces as evidence to solve a homicide |
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| Affiliation: | 1. IGEVET – Instituto de Genética Veterinaria (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, UNLP, La Plata, Buenos Aires, Argentina;2. GECOBI – Grupo de Genética y Ecología en Conservación y Biodiversidad, Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”, Av. Angel Gallardo 470, C1405DJR Buenos Aires, Argentina;1. Research Institute of Ophthalmology, Cairo, Egypt;2. Division of Ophthalmology, Department of Clinical Neurosciences, Geneva University Hospitals, Geneva, Switzerland;3. Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, California States;1. First Department of Forensic Science, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan;2. Forensic Science Laboratory, Kyoto Prefectural Police Headquarters, 85-3, 85-4 Shimodachiuri-dori, Kamanza-higashiiru, Yabunouchi-cho, Kamigyo-ku, Kyoto 602-8550, Japan;3. Identification Center, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan;1. Department of Surgery, St. Paul''s Hospital & University of British Columbia, Vancouver, BC, Canada;2. Department of Radiation Oncology, BC Cancer Agency, Vancouver, BC, Canada;3. Michael Smith Genome Sciences Centre, Vancouver, BC, Canada;4. Functional Imaging Department, BC Cancer Agency, Vancouver, BC, Canada |
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| Abstract: | Dog fecal samples were collected at the crime scene and from the shoes of the suspect to see whether they could be linked. DNA was genotyped using a 145 bp fragment containing a 60 bp hotspot region of the mitochondrial DNA (mtDNA) control region. Once the species origin was identified, sequences were aligned with the 23 canine haplotypes defined, showing that evidence and reference had 100% identity with haplotype 5. The frequency of haplotype 5 and the exclusion power of the reference population were 0.056 and 0.89, respectively. The forensic index showed that it was 20 times more likely that the evidence belonged to the reference dog than to some other unknown animal. The results support that the mtDNA hypervariable region 1 (HV1) is a good alternative for typing in trace or degraded casework samples when the STR panel fails, and demonstrate the utility of domestic animal samples to give additional information to solve human legal cases. |
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| Keywords: | Forensic sciences Non-human DNA Dog Mitochondrial DNA Feces |
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