| 棘阿米巴土壤分离株CB/S1的线粒体DNA限制性片段长度多态性 |
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| 引用本文： | 崔春权,玄英花,郑善子,金松竹,杨香. 棘阿米巴土壤分离株CB/S1的线粒体DNA限制性片段长度多态性[J]. 现代预防医学, 2012, 39(4): 972-973,976 |
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| 作者姓名： | 崔春权 玄英花 郑善子 金松竹 杨香 |
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| 作者单位： | 1. 延边大学基础医学院免疫学与病原生物学教研部,延吉,133002
2. 延边大学工学院计算机科学与技术系 |
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| 摘 要： | [目的]观察棘阿米巴土壤分离株CB/S1的线粒体DNA限制性片段长度多态性(RFLP)。[方法]从棘阿米巴CB/S1提取线粒体DNA用EcoRI酶切,与土壤分离株CJY/S4比较观察限制性片段长度多态性。用地衣红-卡红染色观察棘阿米巴内共生细菌。[结果]棘阿米巴土壤分离株CB/S1线粒体DNARFLP与CJY/S4相比除了额外的片段(extra bands),两个分离株具有相同的片段模式。经地衣红染色观察CB/S1内共生细菌呈黑色、棒状、不规则的分布在胞质内。[结论]额外的片段表明棘阿米巴CB/S1内含有内共生细菌的环状DNA,并且除了内共生细菌的存在棘阿米巴CB/S1与土壤分离株CJY/S4具有密切的亲缘关系。线粒体DNA限制性片段长度多态性是棘阿米巴分类及发现内共生细菌的简便而有效的方法。
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| 关 键 词： | 棘阿米巴 内共生细菌 限制性片段长度多态性 |
MITOCHONDRIAL DNA RESTRICTION FRAGMENT LENGTH POLYMORPHISM(RFLP)OF ACANTHAMOEBA SP.CB/S1 ISOLATED FROM SOIL |
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| Affiliation: | CUI Chun-quan,XUAN Ying-hua,ZHENG Shan-zi,et al.(Department of Pathogenic Biology,Yanbian University College of Basic Medicine,Yanji 133000,China) |
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| Abstract: | [Objective]To survey characterization of Mitochondrial DNA Restriction Fragment Length Polymorphism(MtDNA RFLP)of Acanthamoeba sp.CB/S1 isolated from soil of china.[Methods]The extracted MtDNA was digested with the Eco RI restriction enzyme,electrophoresed in 0.7% agarose gel.The endosymbiont of Acanthamoeba sp.was characterized with orcein-carmime staining[Results]0Acanthamoeba sp.CB/S1 showED extra bands as well as common bands with the other Acanthamoeba sp.CJY/S4 strain isolated from soil.rode-shaped endosymbionts were randomly distributed within the cytoplasm of trophozoite of Acanthamoeba sp.CB/S1.[Conclusion]These results indicated that the.Acanthamoeba sp.CB/S1 may possibly harbor intracellular endosymbiotic bacteria containing circular DNAs.With the exception of the presence of endosymbionts,Acanthamoeba sp.CB/S1 with Acanthamoeba CJY/S4 were found to be closely phylogenetically related. |
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| Keywords: | Acanthamoeba Endosymbiont Restriction fragment length polymorphism |
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