|
|||||
|
|
Inhibitory effect of Uncaria sinensis on human aortic smooth muscle cell migration is based on matrix metalloproteinase-9 inhibitory activity |
|
| Authors: | Ha Ki-Tae Kim Kyung-Woon Suh Seok-Jong Kwak Chang-Geun Kim June-Ki Kim Cheorl-Ho |
| Affiliation: | aDepartment of Pathology and Cardiovascular MRC, Dongguk University, Kyungju 780-714, South Korea bDepartment of Biological Science, SungKyunKwan University, Chunchun-Dong, Suwon City, Kyunggi-Do 440-746, South Korea |
| Abstract: | Medicinal extracts of Cho-Deung-san and Uncaria sinensis Havil. (UR) have previously been shown to have inhibitory effects on migration of vascular smooth muscle cells (VSMC) and matrix metalloproteinase (MMP)-2/9 production, which play key roles in the development of atherosclerosis. In this study, we have more extensively investigated the inhibitory effect of UR on MMP-9 activity and TNF- induced human aortic smooth muscle cells (HASMC) migration. The result from gelatin zymography showed that UR inhibited MMP-9 activity in a dose-dependent manner (IC50 = 55 μg/ml). In addition, UR strongly inhibited the migration of HASMC induced by TNF- treatment (IC50 = 125 μg/ml), although it has very low cytotoxic effect on HASMC (IC50 > 500 μg/ml). These results suggest that UR is a potential anti-atherosclerotic agent through inhibition of MMP-9 activity and VSMC migration. |
| Keywords: | Uncaria sinensis Human aortic smooth muscle cells Migration Matrix metalloproteinase-9 Zymography |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |