突聋患者血浆内皮素和血清一氧化氮的测定

目的研究内皮素(ET)和一氧化氮(NO)在突发性感音神经性聋的发生、发展及预后中的作用,探讨突聋的发病机制,为临床诊治突聋及判断预后提供理论依据。方法对2002-08～2003-02长沙地区26例突聋患者进行血浆ET和血清一氧化氮代谢产物硝酸盐的含量测定,并同期对18例健康对照者进行测定。结果突聋患者治疗前ET水平明显高于健康对照者,而NO水平明显低于健康对照者(P<0.05);治疗有效组治疗后ET水平明显降低达基本正常水平(P<0.05),而NO水平明显提高,但仍低于健康对照者(P<0.05);治疗有效组治疗前ET水平明显低于无效组,NO明显高于无效组,治疗后亦如此(P<0.05);不同程度耳聋组之间ET和NO水平无显著性差别(P>0.05)。结论血浆ET水平增高和血清NO水平减低在突聋的发生、发展过程中可能起着一定的作用;血浆ET水平增高程度和血清NO水平减低程度与突聋患者的耳聋程度无关,但对判断其预后可能起着重要作用。

Detection of Plasma Endothelins and Serum Nitric Oxide in the Patients with Sudden Sensorineural Hearing Loss

Objective To investigate the roles of endothelins and nitric oxide in the pathogenesis of sudden sensorineural hearing loss(SSHL). Methods 26 patients with SSHL in Changsha area from October 2000 to April 2001, and 18 healthy subjects in the same period were enrolled in this study. Plasma endothelins level was detected by radioimmunoassay, and serum nitric oxide(NO) level was detected by cadmium-reduced spectrophotometry. Results The plasma endothelins level in the SSHL patients was significantly higher than that in the healthy subjects, and the serum NO level in the SSHL patients was significantly lower than that in the healthy subjects(P<0.05). After treatment, the plasma endothelins level decreased, and the NO level increased significantly in the hearing improved patients(P<0.05). Plasma endothelins increase and NO level decrease in the hearing unimproved patients were more obvious than those in the hearing improved patients either before or after treatment(P<0.05), but there was no significant difference in the endothelins and NO level among the SSHL patients with different hearing level (P>0.05). Conclusion The increase of ET level and the decrease of NO level may play an important role in the pathogenesis of SSHL, which may serve as an index to predict the hearing improving or not, but were not related to the degree of hearing loss.