| 脐血单个核细胞体外向神经干细胞的诱导及Foxg1基因的表达 |
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| 引用本文： | 范亚珍,王军,陈海,朱登纳,吴值荣,侯艳艳.脐血单个核细胞体外向神经干细胞的诱导及Foxg1基因的表达[J].山东医药,2010,50(11):13-15. |
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| 作者姓名： | 范亚珍 王军 陈海 朱登纳 吴值荣 侯艳艳 |
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| 作者单位： | 郑州大学第三附属医院,郑州,450052 |
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| 基金项目： | 河南省医学科技攻关计划项目 |
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| 摘 要： | 目的探讨人脐血单个核细胞（UBC-MNCs）体外向神经干细胞（NSCs）的诱导分化机制及NSCs中Foxg1基因表达的意义。方法从脐血中分离出UBC—MNCs，用含hEGF、bFGF和B27因子的Neurobasa1培养基联合诱导其向NSCs方向分化，观察NSCs形态学及增殖分化特点；免疫组化法检测培养细胞中神经细胞标志抗原Nestin、NSE、GFAP的表达情况，BrdU标记靶细胞；RT-PCR法检测分离后、培养3、6、9、12d细胞中Foxg1基因的表达。结果UBC—MNCs可定向诱导分化为神经元样细胞，表达Nestin、NSE、GFAP标记抗原。Foxg1 mRNA在诱导前细胞中低表达，诱导后逐渐升高（P〈0．05），6d达高峰，之后逐渐下降（P〈0．05）。结论体外定向诱导培养可获得UBC-MNCs源性NSCs，Foxg1基因是NSCs增殖分化关键调控因子，脐血可成为NSCs的新来源。
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| 关 键 词： | Foxg1基因 脐血 神经干细胞 细胞培养 诱导分化 |
Induction and Foxg1 gene expression of the neural stem cells from human umbilical cord blood mononuclear cells in vitro |
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| FAN Ya-zhen,WANG Jun,CHEN Hai,ZHU Den-gna,WU Zhi-rong,HOU Yan-yan.Induction and Foxg1 gene expression of the neural stem cells from human umbilical cord blood mononuclear cells in vitro[J].Shandong Medical Journal,2010,50(11):13-15. |
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| Authors: | FAN Ya-zhen WANG Jun CHEN Hai ZHU Den-gna WU Zhi-rong HOU Yan-yan |
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| Institution: | FAN Ya-zhen,WANG Jun,CHEN Hai,ZHU Deng-na,WU Zhi-rong,HOU Yan-yan(The Third Hospital Affiliated to Zhengzhou University,Zhengzhou 450052,P.R.China) |
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| Abstract: | Objective To explore the mechanism of human umbilical cord blood mononuclear cells(UBC-MNCs) inducing and differentiating into neural stem cells(NSCs) in vitro and the significance of Foxg1 gene expression.Methods The UBC-MNCs were isolated from human umbilical cord blood,induced and diferentiated into NSCs in Neurobasal medium containing hEGF,bFGF and B27 factures in vitro,the characteristics of morphology and proliferation,differentiation were observed;the expression of specific neuron antigen markers of ... |
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| Keywords: | Foxg1 gene umbilical cord blood neural stem cells cell culture induction and differentiation |
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