| 应用两种方法进行无损伤性产前诊断研究 |
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| 引用本文： | 覃建庆,马洪星,汪晶冰,宋一丽,王成英,宋全春. 应用两种方法进行无损伤性产前诊断研究[J]. 医学研究杂志, 2003, 32(12): 46-47 |
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| 作者姓名： | 覃建庆 马洪星 汪晶冰 宋一丽 王成英 宋全春 |
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| 作者单位： | 黑龙江省大庆油田总医院,163000 |
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| 摘 要： | 目的用两种不同的方法进行胎儿性别鉴定,分析二者有无明显差异。方法从孕妇外周血分离血浆和胎儿有核红细胞(Nucleated red blood cells,NRBCs),应用套式PCR特异性扩增DYS1片段,琼脂糖凝胶电泳检测结果。结果 30例受检者中,共有男要18例,血浆组检出率为88.9％(16/18);有核红细胞组检出率为66.7％(12/18)。
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| 关 键 词： | 无损伤性产前诊断 血浆 有核红细胞 流式细胞术 |
| 修稿时间： | 2003-07-01 |
Application of two methods in noninvasive prenatal diagnosis |
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| Qin Jianqing,Ma Hongxing,Wang Jingbing,et al.. Application of two methods in noninvasive prenatal diagnosis[J]. Journal of Medical Research, 2003, 32(12): 46-47 |
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| Authors: | Qin Jianqing Ma Hongxing Wang Jingbing et al. |
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| Affiliation: | Qin Jianqing,Ma Hongxing,Wang Jingbing,et al.Daqing First Hospital Daqing 163000,China |
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| Abstract: | Objectve To identify the sex of fetus with two approaches, and find out the difference of them. Methods Separating the plasma and sorting the fetal nucleated red Hood cells (NRBCs) from the blood of pregnant women.The DYS1 gene was amplified with nest PCR.Agarose gel electrophoresis was used to test the results. Result There are 18 male fetuses in 30 pregnant women. In group of plasma, male pregnancy was correctly predicted in 88.9% (16/18); in group of NRBCs, the agreement was 66.7% (12/18) .There is not significant difference between two approaches. |
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| Keywords: | Noninvasive prenatal diagnose Blood plasma Nucleated red blood cells Flow cytometry |
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