一种改良的小鼠原代肝细胞培养方法

为寻求一种简易、价廉的原代小鼠肝细胞培养方法 ,采用非灌注法、冰浴操作处理肝组织块及改良低血清培养基进行了原代鼠肝组织块和鼠肝细胞单层培养。通过光镜观察细胞形态、电镜观察超微结构及检测培养上清白蛋白水平证实培养细胞为肝细胞。持续培养结果显示原代培养第 6～ 12 d左右为肝细胞功能最佳观察和实验阶段。

An Improved Method for the Primary Culture of Murine Hepatocytes

To search for an easier and a cheaper method for the primany culture of murine hepatocytes, the primary culture of murine liver tissue and monolayer hepatocytes was performed in a simple way of non perfusion under the ice bath treating the live tissues with a modified low serum medium. The cultured cells were proved to be hepatocytes by observing morphology and structure under the light microscopy and the electron microscopy as well as by detecting album levels in the supernatant of the cultures. The results showed that the duration from the 6th to 12th d of the primary culture was the optium period to carry out research work on the hepatocytes.