Twist 基因联合p53E-cadherin检测在食管癌中的意义*

目的:检测食管癌患者的癌组织、癌旁组织和正常食管组织中Twist、p53、E-cadherin 蛋白表达水平，探讨Twist蛋白表达与食管癌临床病理参数的关系，并阐述Twist与p53、E-cadherin 蛋白表达的相关性。方法:各选取30例食管癌组织和癌旁组织，同时选取10例良性食管疾病的正常食管组织，应用免疫组化和免疫印迹方法进行蛋白检测，实验结果应用SPSS15.0 统计软件包进行数据分析处理。结果:Twist、p53、E-cadherin 蛋白表达水平在食管癌组织、癌旁组织和正常食管组织中有明显差异；在不同浸润深度、有无淋巴结转移、TNM分期及肿瘤分化程度不同的食管癌组织Twist蛋白表达具有明显差异；食管癌组织中Twist表达水平与p53、E-cadherin 之间存在一定的相关性。结论:Twist可能通过调节ARF/p 53途径抑制细胞凋亡促进肿瘤细胞形成，同时影响E-cadherin 的表达而促进上皮- 间质转化现象，参与食管癌的发生和转移，其可能成为食管癌诊断的分子生物学指标和分析食管癌患者病程以及判断疾病预后的特异性指标之一。 

Significance of Twist,p53 and E-cadherin Protein Expression in Esophageal Cancer

Objective: To detect the level of Twist, P53, and E-cadherin protein expression in human esophageal can-cer tissue, adjacent tissue and normal esophageal tissue, and to determine if there is a relevant relationship between Twist, P53, and E-cadherin protein expression and the clinicopathological parameters of esophageal cancer. Methods:Research-ers collected 30samples of esophageal cancer tissue and adjacent tissue and 10normal esophageal tissue samples from patients with benign esophageal disease. Immunohistochemistry and Western blot were used to detect the expression of Twist, P53and E-cadherin. The results were analyzed by SPSS 15.0. Results: The expression of Twist and P 53was higher in esophageal cancer tissue than in adjacent tissue and normal esophageal tissue. E-cadherin expression was lower in esophageal cancer tissue than in adjacent tissue and normal esophageal tissue. No significant difference was found in Twist, P53and E-cadherin expression between adjacent tissue and normal esophageal cancer tissue. Twist protein expres-sion was significantly correlated with depth of invasion, lymph node metastasis, TNM stage and tumor differentiation. Conclusion: Twist may inhibit cell apoptosis through regulation of the ARF/P 53pathway and can affect E-cadherin expression, promoting the epithelial-mesenchymal transition (EMT) and promoting the occurrence and metastasis of esophageal can-cer. Twist can be used as a specific index for the diagnosis and prognostic evaluation of esophageal cancer.