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| 基于CO1基因序列的DNA条形码在中尼边境鼠类物种鉴定中的应用 | |
| 引用本文： | 陈春生,张晓龙,张雪莲,范泉水,牛文忠,胡小兵,格龙,房键慧,曹晓梅,潘其礼,王静. 基于CO1基因序列的DNA条形码在中尼边境鼠类物种鉴定中的应用[J]. 西南国防医药, 2012, 22(1): 22-24. DOI: 10.3969/j.issn.1004-0188.2012.01.007 |
| 作者姓名： | 陈春生 张晓龙 张雪莲 范泉水 牛文忠 胡小兵 格龙 房键慧 曹晓梅 潘其礼 王静 |
| 作者单位： | 1. 西藏出入境检验检疫局,拉萨,850002 2. 中国检验检疫科学研究院 3. 成都军区疾病预防控制中心 4. 西藏地方病防治所 |
| 摘 要： | 目的 探讨了线粒体CO1基因作为DNA条形码对鼠类进行物种鉴定的可行性.方法 将从中尼边境采集到的42个鼠类标本,提取基因组DNA,用通用引物PCR法扩增线粒体CO1基因,并测序.将测序结果与GenBank中其他鼠类物种的DNA条码进行BLAST比对,并构建分子进化树.结果 本研究中有17个标本能通过PCR扩增出特异性CO1基因条带,17个标本的种内遗传距离平均值为0.035,种间遗传距离平均值为0.47,种间遗传距离是种内遗传距离的10倍以上.根据BLAST比对法,将采集到的17个标本分到不同的鼠种中.结论 CO1基因序列能够对鼠类进行有效的物种鉴定. |
| 关 键 词： | 鼠类分类 CO1基因 DNA条形码 分子进化树 |
Application of DNA bar code based on CO1 gene order in identification of murid species in Sino-Nepali border area |
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| Chen Chunsheng,Zhang Xiaolong,Zhang Xuelian,Fan Quanshui,Niu Wenzhong,Hu Xiaobing,Ge Long,Fang Jianhui,Cao Xiaomei,Pan Qili,Wang Jing. Application of DNA bar code based on CO1 gene order in identification of murid species in Sino-Nepali border area[J]. Medical Journal of National Defending forces in Southwest China, 2012, 22(1): 22-24. DOI: 10.3969/j.issn.1004-0188.2012.01.007 | |
| Authors: | Chen Chunsheng Zhang Xiaolong Zhang Xuelian Fan Quanshui Niu Wenzhong Hu Xiaobing Ge Long Fang Jianhui Cao Xiaomei Pan Qili Wang Jing |
| Affiliation: | 1. Entry - Exit Inspection and Quarantine Bureau of Tibet, Lhasa,Tibet,850002, China ;2. Inspection and Quarantine Science Institute of China, Beijing, 100123, China;3. Center for Control and Prevention of Diseases of Chengdu Military Command,Chengdu,Sichuan,610021 ,China;4. Dispensary of Endemic Diseases in Tibet,Lhasa,Tibet,850000,China) |
| Abstract: | Objective To discuss the feasibility of using mitochondrial CO1 gene as DNA bar code to identify species of murid. Methods The genome DNA was extracted from 42 murid samples collected in the Sino - Nepali border area. The mitochondrial COl gene was amplified by method of consensus primer PCR and sequenced. BLAST comparison was made between the sequencing results and the DNA bar codes of other murid species in genebank,and molecular evolution tree was built. Results In this research, specific CO1 gene straps in 17 samples were amplified by PCR. The average intraspecies genetic distance of the 17 samples was 0. 035. The average interspecies genetic distance was 0.47. The interspecies genetic distance was almost 10 times more than the intraspecies genetic distance. According to the BLAST comparison,the 17 samples were classified in different murid species. Conclusion The CO1 gene order can be used effectively to identify the murid species. |
| Keywords: | classification of murid CO1 gene DNA bar code molecular evolution tree |
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