芒果皮提取物的体外清除自由基作用研究

目的:对芒果皮提取物的体外清除自由基作用进行研究,为芒果皮的开发利用提供理论依据。方法:分别以水、95%乙醇和石油醚为溶剂提取芒果皮有效成分,再采用紫外-可见分光光度法研究芒果皮3种溶剂提取物对DPPH自由基、超氧阴离子自由基和羟自由基的清除作用。结果:芒果皮的水提取物、95%乙醇提取物和石油醚提取物对上述3种自由基均有很好的清除作用,并且清除率与样品浓度呈现一定的线性关系,浓度越高,清除作用越强;3种提取物对自由基的清除能力为95%乙醇提取物>水提取物>石油醚提取物。结论:芒果皮提取物对自由基有良好清除作用,在药品和保健品方面具有广阔的开发利用前景。

Study on Free Radical Scavenging in Vitro by Mango Peel Extracts

Objective: To study the effect of mango peel extracts on free radical scavenging in vitro and provide a theoretical basis for the development and utilization of the mango peel.Methods: The active ingredients of the mango peel was first extracted by water,95% of ethanol and petroleum ether,and then UV-visible spectrophotometry was used to study the scavenging effects of the mango peel extracts in the three solvents on DPPH radical,superoxide anion radical and hydroxyl radical.Results: The aqueous extract,the 95% ethanol extract and the petroleum ether extract of the mango peel showed good scavenging effects on the three above free radicals.The clearance rate showed a certain linear relationship with sample concentration: the higher the concentration,the stronger the scavenging effect.The free radical scavenging capacity of the three extracts was 95% of ethanol extract>water extract>petroleum ether extract.Conclusion: The mango peel extracts have a good scavenging effect on the free radicals,showing a broad prospect for the development and utilization of the mango peel in medicine and health care products.