FasL基因关节腔内转染对佐剂性关节炎大鼠滑膜细胞凋亡的影响

目的观察FasL基因关节腔内转染对佐剂性关节炎大鼠滑膜细胞的凋亡诱导作用。方法 SD大鼠分为正常转染对照组、正常转染组、模型转染对照组、模型转染组。向模型转染对照组、模型转染组动物右后足趾皮内注射0.1 mL弗氏完全佐剂致炎,诱导佐剂性关节炎。观察大鼠关节炎指数,大鼠滑膜细胞增生、炎性细胞浸润情况,检测滑膜细胞凋亡水平。结果模型转染组与模型转染对照组比较大鼠关节炎指数(AI)显著降低,差异有统计学意义(P<0.05);模型转染对照组、正常转染组与正常转染对照组比较,大鼠滑膜细胞凋亡指数差异无统计学意义(P>0.05),模型转染组与正常转染对照组、模型转染对照组比较,大鼠滑膜细膜凋亡指数间差异有统计学意义(P<0.01)。结论 FasL基因关节腔内转染可诱导佐剂性关节炎大鼠滑膜细胞凋亡,降低佐剂性关节炎大鼠的关节炎指数。

Study of Effects of Direct Intra-articular Injection of Adenovirus-Fasl on Apoptosis of Synoviocytes in Adjuvant Arthritis Rats

Objective To observe the effects of direct intra-articular injection of Adenovirus-FasL(Ad-FasL)on apoptosis of synoviocytes in adjuvanpt arthritis(AA)rats.Methods Rats were averagely divided into four groups:normal Ad-LacZ control group,normal Ad-FasL group,model Ad-LacZ control group and Amodel Ad-FasL group.Amodel Ad-LacZ control group and Amodel Ad-FasL group were intracutaneously injected with 0.1 mL of Freund′s complete adjuvant in the right hinglimb.The arthritis index(AI),synovial cell hyperplasia,inflammatory cell infiltration and the synoviocytes apoptosis index were assayed.Results Compared with model Ad-LacZ control group,AI of the model AdFasL group decreased(P<0.05);the apoptosis index of synoviocytes between normal Ad-LacZ control group,normal Ad-FasL group and model Ad-LacZ control group has no significant difference(P>0.05).Compared with normal Ad-LacZ control group and model Ad-LacZ control group,the apoptosis index of model Ad-FasL group increased(P<0.01).Conclusion Intra-articular injection of Ad-FasL can enhance apoptosis index of synoviocytes as well as decrease AI of rats.