脑梗死患者载脂蛋白AI基因多态性分布的病例对照研究

目的 观察载脂蛋白ＡＩ基因多态性在动脉粥样硬化脑梗死患者中的分布，探讨基因型与脑梗死的关系。方法 用聚合酶链反应技术（ＰＣＲ）检测北京地区１９９例脑本钱 ２０４例健康人的载脂蛋白ＡＩ基因启动子和第一内含子两个ＭｓｐＩ酶发位点限制性片段长度多态民生（ＲＬＦＰ）。结果 北京地区４０３例受检者中载脂蛋白ＡＩ基因启动子均以Ｍ１＋＝和Ｍ１＋－为主要基因型，第一内含子大多数为Ｍ２＋＋基因型；脑梗组ＭＡ＋－基因

A case-control study on the distribution of apolipoprotein AI gene polymorphisms in the survivors of atherosclerosis cerebral infarction

OBJECTIVE: To investigate the frequency of the restrict fragment length polimorphisms (RFLP) at 2 MspI sites in the 5' - end of apolipoprotein AI (ApoAI) gene and its potential interaction to atherosclerotic cerebral infarction (ACI) in Chinese Han population. METHOD: Polymerase chain reaction (PCR) technique was used in a sample of 199 cases with documented ACI and 204 healthy matched by age and sex individuals selected from Chinese Han nationality in Beijing. The studied loci include promoter region (-75 bp) and the intron - 1 (+83 bp). RESULTS: (1) Both ACI and control groups M1 + + and M1 + - were major frequent genotypes in Beijng area, M2 + + were major frequent genotypes in intron - 1; (2) M1 + - genotype appeared more common among patients than in controls (0.487 vs 0.368, P < 0.05, OR = 1.64); (3) Individuals with M1 + -/M2 + + haplotype were significantly increased in patients compared with controls (0.437 vs 0.314, P < 0.05, OR = 1.70). CONCLUSION: It is suggested that both M1 + - genotype and M1 + -/M2 + + haplotype of ApoAI gene might associate with ACI in our study.