人脐血CD34+细胞体外DEXTER基质细胞培养的动态观察

目的 动态观察人脐血CD34^ 细胞DEXTER基质细胞培养的情况。方法 应用MACS磁珠分离系统分离脐带血CD34^ 细胞，按照DEXTER法行脐血基质细胞培养，观察不同时间、不同细胞因子组合基质细胞的生长状态。结果 SCF及SCF BFGF组合可促进基质细胞集落的生成，脐血基质细胞在生长情况、组成成分、细胞形态等方面与骨髓基质细胞有不同的生物学特性。结论 脐血CD34^ 细胞在生长因子的辅助下能促进基质细胞集落的形成，有望成为新的基质细胞来源。

Dynamic observation of the DEXTER culture of cord CD34+ cells in vitro

Objective To observe dynamically the DEXTER culture of cord CD34 + cells in vitro . Methods CD34 + cord cells were separated by MACS system from 9 fresh samples. Stromal cells in umbilical blood were cultured by using DEXTER culture system. After adding the different cytokine(s)individually, the number of cord stromal cell colony was counted and the morphology of the cord stromal cells in culture was observed respectively at different time points. Results SCF+BFGF, SCF could promote the growth of cord stromal cell colony. The biological properties of cord stromal cells were different from those of bone marrow stromal cells at many aspects such as growth condition, conformation, cell morphology, etc . Conclusion Cord CD34 + cells with the aid of some hematopoietic growth factors have promoting effects on the expansion of cord stromal cells and may become a new resource of stromal cells.