肌萎缩侧索硬化与血浆同型半胱氨酸关系的研究

目的研究肌萎缩侧索硬化与同型半胱氨酸水平的关系。方法采用断面研究，21例肌萎缩侧索硬化患者为ALS组，选取40例健康者为对照组，及检测两组患者的Hey、叶酸、维生素B12,用高效液相色谱仪和荧光检测仪测定两组血浆总Hey水平。结果Hey水平肌萎缩侧索硬化组17．65[17．65±11．23]μmol／L显著高于对照组10．32[10．32±6．72]μmol／L；肌萎缩侧索硬化组[9．52±2．57]ng／mL叶酸水平明显低于对照组13．56[13．56±3．23]ng／mL；维生素B12水平在两组间无统计学差异（P〉0．05），肌萎缩侧索硬化组为（651±112．34）pg／mL，对照组为（632±164．89）pg／mL。结论Hey水平升高与肌萎缩侧索硬化密切相关，可能参与了肌萎缩侧索硬化的病理生理过程。

Study on Relation between Plasma Level of Homocysteine and amyotrophic lateral sclerosis

Objective To examine the relationship between Hcy levels and clinical ALs.Methods In a cross-sectional study,21 amyotrophic lateral sclerosis patients were enrolled as ALS group and 40 healthy person as control group.The plasma Ievek of Hcy,B1,and fohte levels were measured in all subjects.Hcy levels were measured using highpefformance liquid chromatography with fluorescence detection.Results Patients with ALS had higher median plasma Hcy levels 17.65(17.65 ±11.23]us 10.32[10.32 4-6.72]μmol/L;P＜0.01)and lowerfolate levels9.52(9.52±2.57]us 13.56[13.56 4-3.23]ng/mL;P＜0.01),compared with controls.no significant difference was observed in plasma B12 levels between the two groups,651±112.34 pg/mL in ALS group,632±164.89 pg/mL in control group.Conclusion High plasma homocysteine(Hcy)levels was related with amyotrophic lateral sclerosis(ALS)compared with healthy controls and may be linked to pathophysiologic progression of the disease.