| 藻酸盐单克隆抗体的制备及鉴定 |
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| 引用本文： | 王睿,柴栋,裴斐,方翼,朱曼.藻酸盐单克隆抗体的制备及鉴定[J].军医进修学院学报,2004,25(5):353-354. |
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| 作者姓名： | 王睿 柴栋 裴斐 方翼 朱曼 |
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| 作者单位： | 解放军总医院,药材处临床药理研究室,北京,100853 |
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| 基金项目： | 国家自然科学基金资助项目 ( 3 0 0 70 72 0 ) |
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| 摘 要： | 目的：制备、筛选稳定分泌藻酸盐单抗的杂交瘤克隆。方法：应用藻酸盐BSA程序免疫BALB／C小鼠，将其脾脏与骨髓瘤细胞SP2／0融合，采用采用ELISA方法筛选阳性克隆。抗体的检测分别采用琼脂糖双向扩散试验和ELISA方法。结果：得到两株分泌藻酸盐单抗的杂交瘤克隆，分别分泌IgM和IgG。
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| 关 键 词： | 藻酸盐 分泌 杂交瘤 单抗 单克隆抗体 ELISA方法 脾脏 阳性克隆 筛选 BSA |
| 文章编号： | 1005-1139(2004)05-0353-02 |
| 修稿时间： | 2004年2月17日 |
Prepareation and identification of monoclonal antibody of alginate |
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| WANG Rui,CHAI Dong,PEI Fei,FANG Yi,ZHU Man.Prepareation and identification of monoclonal antibody of alginate[J].Academic Journal of Pla Postgraduate Medical School,2004,25(5):353-354. |
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| Authors: | WANG Rui CHAI Dong PEI Fei FANG Yi ZHU Man |
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| Abstract: | Objective: To prepare and identify the hybridome clones of monoclonal antibody of alginate. Methods: The spleen cell was obtained from BALB/C by programmed immunization , and then was fused with myeloma cell line SP2/0. The positive clones were obtained by ELISA method. Antibody was detected by double agar gel immune diffusion test and ELISA. Results: Two kinds of hybridome clones of monoclonal antibody of alginate was obtained , which secrete IgM or IgG respectively. |
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| Keywords: | alginate monoclonal antibody ELISA method |
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