Endothelin Potentiates Growth Factor-stimulated DNA Synthesis in Swiss 3T3 Cells |
| |
| Authors: | Masatoshi Kusuhara Ken Yamaguchi Ai Ohnishi Kaoru Abe Satoshi Kimura Hideto Oono Shingo Hori Yoshiro Nakamura |
| |
| Affiliation: | Growth Factor Division, National Cancer Center Research Institute, 5-1-1, Tsukiji, Chuo-ku, Tokyo 104;Department of Medicine, National Cancer Center Hospital, 5-1-1, Tsukiji, Chuo-ku, Tokyo 104;Department of Infectious Diseases and Applied Immunology, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108;Tsukuba Medical Development Center, Mitsubishi Petrochemical Co. Ltd., 8-5-1, Chuo, Amimachi, Inashiki-gun, Ibaraki 300-03;Department of Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160 |
| |
| Abstract: | A murine cell line, Swiss 3T3, is commonly used for the study of cellular growth. The present study revealed that this cell line possesses high-affinity receptors for endothelin, a vasoactive peptide derived from vascular endothelial cells. In this cell line, endothelin potentiated DNA synthesis stimulated by platelet-derived growth factor, basic fibroblast growth factor or insulin. The endothelin concentration required for potentiating DNA synthesis in this cell line is almost the same as that reported previously in endothelin-induced vasoconstriction. Since endothelin increased intracellular calcium levels, this ion may participate in the signal transduction pathways by which endothelin induces potentiation of DNA synthesis. |
| |
| Keywords: | Endothelin Swiss 3T3 cells Growth factor Intracellular calcium Receptor |
|
|
