C57-TgN(HBVadr2.0)SMMU转基因小鼠肝脏的组织病理学观察

目的:研究乙型肝炎转基因小鼠品系C57-TgN(HBVadr2.0)SMMU肝脏的组织学及免疫病理学特征.方法:以168只SPF级乙肝转基因小鼠及15只正常C57BL/6小鼠为研究对象,取肝组织连续切片,常规H-E染色,并选取20例有明显单个核细胞肝内浸润的肝标本,免疫组化原位鉴定白细胞分化抗原的肝内分布.结果:37.5%的转基因小鼠肝脏出现人慢性轻度乙型肝炎样的病理改变,病变率随月龄增大显著增高;32.7%有更轻微的非特异性炎症反应;肝内浸润的单个核细胞多数为CD3 、CD4 细胞,未发现CD57 、CD8 细胞浸润.结论:乙肝转基因小鼠C57 TgN(HBVadr2.0)SMMU肝脏可出现不同程度的病理改变,与人慢性轻度乙肝组织学相类似.

Pathologic observation on liver of C57-TgN(HBVadr2.0)SMMU transgenic mice

Objective:To observe the histological and immunopathological change of liver in hepatitis B virus transgenic mice C57-TgN(HBV adr 2.0)SMMU. Methods: Totally 168 transgenic mice and 15 normal C57BL/6 mice, raised in SPF grade animal husbandry unit, were chosen for this study. The liver was taken for histopathologic examination under light microscopy after Hematoxylin-Eosin stain. Twenty specimens with intrahepatic mononuclear cell infiltration were chosen to study the immunohistochemical location of leukocyte differentiation antigen. Results: Pathological lesion similar to human chronic hepatitis B was found in 37.5% (63/168) transgenic mice, and 32.7% (55/168) transgenic mice had mild nonspecific abnormalities. The frequency of chronic liver lesion was significantly correlated with age. Most of the infiltrated intrahepatic mononuclear cells were CD3 and CD4 positive lymphocytes. Conclusion: The C57-TgN(HBV adr 2.0)SMMU transgenic mice have some histopathological changes similar to human chronic hepatitis B.