木瓜粉对体外缺氧损伤神经细胞抗氧化能力及凋亡的影响

目的 :　观察木瓜粉 (BN)对缺氧损伤神经细胞抗氧化能力及凋亡的影响。方法 :　采用无血清体外细胞培养方法 ,培养鼠胚大脑神经细胞 ,将细胞分为 4组 ,缺氧 0、0 .1、0 .5mg BN/ml组和非缺氧 0 mg BN/ ml组。将缺氧实验的神经细胞置于缺氧环境造成缺氧损伤 ,再培养第 4d(D4)收集细胞 ,测定生化指标 ,同时进行电镜病理检查。结果 :　缺氧 0 .1 mg BN/ ml组神经细胞特异性烯醇化酶 (NSE)活性和四甲基偶氮唑盐 (MTT)高于缺氧 0 mg BN/ ml组 ,差别有显著性 (P<0 .0 5) ;缺氧 0 .1 mg BN/ ml和 0 .5 mg BN/ ml组神经细胞超氧化物歧化酶 (SOD) (78.65±0 .83,85.55± 2 .93 NU/ mg prot)和谷胱甘肽过氧化物酶 (GSH- Px) (43.36± 8.1 0 ,53.31± 6.1 0U/ mg prot)活性均显著高于缺氧 0 mg BN/ ml组 (SOD 69.1 5± 9.30 NU/ mg prot;GSH- Px 2 1 .1 3± 5.65 U/ mg prot;P<0 .0 5) ,缺氧 0 .1 mg BN/ ml和 0 .5 mg BN/ ml组神经细胞丙二醛(MDA)含量 (1 .48± 0 .32 ,1 .75± 0 .2 5 nmol/ mg prot)显著低于缺氧 0 mg BN/ ml组 (2 .64± 0 .39nmol/ mg prot) ,差别有显著性 (P<0 .0 5) ;缺氧 0 .1 mg BN/ ml和 0 .5 mg BN/ ml组的神经细胞凋亡富集系数 (EF,0 .72± 0 .0 9;0 .63± 0 .0 5)低于缺氧 0 mg BN/ ml?

EFFECTS OF PAPAYA ON ANTIOXIDATION AND APOTOSIS OF CEREBRAL NEUROCYTES FOLLOWING ANOXIC DAMAGE IN VITRO

Objective: To study the effects of papaya (BN) on the antioxidation and apoptosis of cerebral neurocytes following hypoxia damage in vitro. Methods: The rat embryonic cerebral neurocytes were cultured with serum-free medium in which BN was added and were divided into four groups (hypoxia+0mg BN/ml, hypoxia+0.1mg BN/ml, hypoxia+0.5mg BN/ml and non-hypoxia +0 mg BN/ml groups). At the end of the experiment, cultured cerebral neurocytes were collected to detect biochemical indices and make a pathological observation by using electronic microscope. Results: The activities of NSE and MTT of neurocytes in hypoxia+0.1 mg BN/ml group were significantly higher than those in hypoxia+0 mg BN/ml group (P<0.05). The activities of SOD and GSH-Px in hypoxia+0.1mg BN/ml and hypoxia+0.5 mg BN/ml groups (SOD 78.65±0.83, 85.55±2.93 NU/mg protein; GSH-Px 43.36± 8.10 , 53.31±6.10 U/ mg protein)were significantly higher than those in hypoxia+0 mg BN/ml group (SOD 69.15±9.30 NU/mg protein; GSH-Px 21.13±5.65 U/ mg protein, P<0.05). Compared with hypoxia+0mg BN/ml group (MDA 2.64±0.39 nmol/mg protein), the level of MDA of hypoxia+0.1 mg BN/ml and hypoxia+0.5 mg BN/ml groups (1.48±0.32, 1.75±0.25 nmol/mg protein) was decreased significantly (P<0.05). The enrichment factor of neurocytes apoptosis in hypoxia+0.1 mg BN/ml and hypoxia+0.5 mg BN/ml groups (0.72±0.09, 0.63±0.05)were significantly lower than those in hypoxia+0mg BN/ml group (1.64±0.16,P<0.05). The morphological observation under electronic microscope showed that the recovery of neurocytes following anoxic damage in hypoxia+0.1 mg BN/ml and hypoxia+0.5 mg BN/ml groups were significantly better than those in hypoxia+0 mg BN/ml group in vitro. Conclusion: Papaya can improve the antioxidative ability and promote the recovery of cerebral neurocytes following anoxic damage in vitro.