Role of vitelline envelope during fertilization in the black tiger shrimp, Penaeus monodon |
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| Authors: | Ngernsoungnern Piyada Ngernsoungnern Apichart Chaiseha Yupaporn Sretarugsa Prapee |
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| Institution: | a School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand
b Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand |
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| Abstract: | Animal eggs possess investments through which sperm must penetrate. The aim of the present study was to investigate the role of the egg coating, the vitelline envelope, during sperm-egg interactions in the black tiger shrimp, Penaeus monodon. The site(s) of primary binding between sperm and egg and the possible binding molecule(s) for sperm were identified. In vitro adsorption of the vitelline envelope protein onto the sperm surface showed that primary binding occurred between the sperm anterior spike of acrosome intact sperm and the vitelline envelope. Results from streptavidin blotting revealed that the component of the vitelline envelope that interacts with the sperm integral membrane protein is a 370 kDa protein. In addition, it was shown that the vitelline envelope protein had no ability to induce acrosome reaction. These results suggest that the function of the vitelline envelope is as a primary binding site for sperm in shrimp, but not a sole trigger for the acrosome reaction. |
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| Keywords: | AR acrosome reaction ASW artificial sea water ECL enhanced chemiluminescence EW egg water NP-40 nonidet-40 PAB p-amino benzamidine TAPS N-tris(hydroxymethyl)methyl-3-amino-propanesulfonic acid TBS Tris buffered saline VE vitelline envelope |
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