|
|||||
|
|
Morphological alterations and distribution of occludin in rat testes after bilateral vasectomy |
|
| Authors: | Gazi ContukOya Orun Emel Demiralp-Ek?io?luFeriha Ercan |
| Institution: | a Department of Histology and Embryology, School of Medicine, Marmara University, Haydarpasa, 34688 Istanbul, Turkey b Department of Biophysics, School of Medicine, Marmara University, Istanbul, Turkey c Department of Hematology and Immunology, School of Medicine, Marmara University, Istanbul, Turkey |
| Abstract: | The aim of study was to investigate the fate and the morphology of the cells which constitute the spermatogenic line, and to determine the distribution of occludin in the testis in adult vasectomized Wistar rats. The rats were divided into two groups: control group (sham-operated) and vasectomized group. One, 3 and 6 months after sham and vasectomy operations, testis samples were examined. The weight of the testes was found to be reduced 3 and 6 months after vasectomy. There was vacuolization in the seminiferous tubules one month after vasectomy. The tubules showed severe atrophy 3 and 6 months after vasectomy. The occludin immunolabeling in the 3- and 6-month groups was weak and diffuse, and the density of the protein was found to be decreased. The increase in the number of apoptotic cells was accompanied by a time-dependent decrease in the number of haploid, diploid and tetraploid cells. This study demonstrated that vasectomy causes degeneration in the seminiferous tubules with alterations in occludin distribution with a decrease in the number of spermatogenic cells. Moreover, these alterations increase in a time-dependent manner. |
| Keywords: | AEC 3-amino-9-ethylcarbazole ANOVA analysis of variance Ca2+ calcium ions cAMP cyclic adenosine monophosphate DNA deoxyribonucleic acid H& E hematoxylin and eosin H2O2 hydrogen peroxide IgG immunoglobulin G kDa kiloDalton kV kiloVolt NIH National Institutes of Health NTB/BCIP nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate PAS periodic acid-Schiff PBS phosphate buffered saline PMSF phenylmethylsulfonyl fluoride SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis SEM standard error of the mean TdT terminal deoxynucleotidyl transferase TEM transmission electron microscopy Tris-HCl 2-amino-2-(hydroxymethyl)-1 3-propanediol hydrochloride TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling ZO-1 Zona occludens-1 ZO-2 Zona occludens-2 |
| 本文献已被 ScienceDirect 等数据库收录！ | |