Expression of macro non-coding RNAs Meg8 and Irm in mouse embryonic development |
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Authors: | Gu Tiantian He Hongjuan Han Zhengbin Zeng Tiebo Huang Zhijun Liu Qi Gu Ning Chen Yan Sugimoto Kenkichi Jiang Huijie Wu Qiong |
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Affiliation: | a State Key Laboratory of Urban Water Resource and Environment, Department of Life Science and Engineering, Harbin Institute of Technology, No. 92 West Da-zhi Street Harbin, 150001, Heilongjiang, China b Department of Cell Science, Faculty of Graduate School of Science and Technology, Niigata University, Niigata, Japan c Cell Therapy Program, Princess Margaret Hospital, Room 4-605, 610 University Avenue, Toronto, ON, M5G, 2M9, Canada d College of Life Sciences, Ludong University, Yantai, 264025, China |
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Abstract: | Non-coding RNAs (ncRNAs) Meg8 and Irm were previously identified as alternatively splicing isoforms of Rian gene. Ascertaining ncRNAs spatiotemporal expression patterns is crucial for understanding the physiological roles of ncRNAs during tissue and organ development. In this study in mouse embryos, we focused on the developmental regulation expression of imprinted macro ncRNAs, Meg8 and Irm by using in situ hybridization and quantitative real-time RT-PCR (QRT-PCR). The in situ hybridization results showed that Meg8 and Irm were expressed in the developing brain at embryonic day 10.5 (E10.5) and E11.5, while Irm expression signals were strikingly detected in the somite, where Meg8 expression signals were undetectable. By E15.5, they were expressed in brain, tongue, liver, lung and neuroendocrine tissues, while Irm displayed more restricted expression in tongue and skeletal muscle than Meg8. Furthermore, quantitative analysis confirmed that they were highly expressed in tongue and brain at E12.5, E15.5 and E18.5. These results indicated that Meg8 and Irm might be coordinately expressed and functionally correlated in diverse of organs. Notably, Irm was more closely associated with morphogenesis of skeletal muscle in contrast to Meg8 during embryonic development. |
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Keywords: | Macro ncRNA Meg8 Irm In situ hybridization QRT-PCR Mouse embryo |
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