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Effects of lycopene and apigenin on human umbilical vein endothelial cells in vitro under angiogenic stimulation |
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| Authors: | Sahin Mehmet Sahin Emel Gümüşlü Saadet |
| Institution: | a Health Sciences Research Centre, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkey b Central Laboratory, Clinical Biochemistry Unit, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkey c Department of Biochemistry, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkey |
| Abstract: | Angiogenesis is the formation process of new blood vessels from preexisting vessels. Solid tumors need angiogenesis for growth and metastasis. The suppression of tumor growth by inhibition of neoangiogenic processes represents a potential approach to cancer treatment. Lycopene has powerful antioxidant capacities and anticarcinogenic properties. The aim of this study was to investigate the effects of lycopene on angiogenesis in vitro. For this reason, we measured in vitro angiogenesis in human umbilical vein endothelial cells including parameters of cell proliferation, tube formation, cell migration. Lycopene and apigenin were observed to block the endothelial cell proliferation in a dose-dependent manner. In addition, they significantly decreased the capillary-like tube lengths, tube formation and endothelial cell migration. This study provides indications that apigenin and lycopene, which are considered as chemopreventive agents, to be effective in vitro on endothelial cells and angiogenesis. |
| Keywords: | BHT butylated hydroxytoluene DMSO dimethyl sulfoxide EBM-2 endothelial basal medium EGM-2 endothelial growth medium EGF epidermal growth factor FBS fetal bovine serum FGF fibroblast growth factor Hep3B a human hepatoma cell line HUVEC human umbilical vein endothelial cells IGF-1 insulin-like growth factor KB-1 a cell line derived from a human oral cavity tumor LNCaP an androgen-sensitive human prostate adenocarcinoma cell line MCF-7 a breast cancer cell line MDA-MB-231 an estrogen-independent human breast cancer cell line MMP matrix metalloproteinase MTT (3&prime (4 5-dimethyl-2-thiazolyl)-2 5-diphenyl-2H-tetrazolium bromide) PC3 a metastatic cell line derived from advanced androgen independent prostate cancer RPMI Roswell Park Memorial Institute cell culture medium SK-Hep1 an immortal and metastatic cell line derived from a liver adenocarcinoma THF tetrahydrofuran VEGF vascular endothelial growth factor |
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