Immunocytochemical analysis of the subcellular distribution of ferritin in Imperata cylindrica (L.) Raeuschel, an iron hyperaccumulator plant |
| |
Authors: | Vicenta de la Fuente |
| |
Institution: | a Departamento de Biología, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain b Centro de Astrobiología (INTA-CSIC), 28850 Torrejón de Ardoz, Madrid, Spain c Centro de Biología Molecular Severo Ochoa (UAM-CSIC), Cantoblanco, 28049 Madrid, Spain |
| |
Abstract: | Ferritin is of interest at the structural and functional level not only as storage for iron, a critical element, but also as a means to prevent cell damage produced by oxidative stress. The main objective of this work was to confirm by immunocytochemistry the presence and the subcellular distribution of the ferritin detected by Mösbauer spectroscopy in Imperata cylindrica, a plant which accumulates large amounts of iron. The localization of ferritin was performed in epidermal, parenchymal and vascular tissues of shoots and leaves of I. cylindrica. The highest density of immunolabeling in shoots appeared in the intracellular space of cell tissues, near the cell walls and in the cytoplasm. In leaves, ferritin was detected in the proximity of the dense network of the middle lamella of cell walls, following a similar path to that observed in shoots. Immunolabeling was also localized in chloroplasts. The abundance of immunogold labelling in mitochondria for I. cylindrica was rather low, probably because the study dealt with tissues from old plants. These results further expand the localization of ferritin in cell components other than chloroplasts and mitochondria in plants. |
| |
Keywords: | Imperata cylindrica Ferritin Jarosite Immunocytochemistry Cell wall Plastid Iron |
本文献已被 ScienceDirect 等数据库收录! |
|