Nonidet P-40致流感病毒脱包膜的AFM观察研究

目的 利用原子力显微镜(AFM)观察经非离子表面活性剂Nonidet P-40(NP-40)不同浓度系列处理的A型流感病毒表面形态变化,观察不同表面活性剂-病毒表面相互作用情况,以提供一种较为温和的病毒表面裂解条件,为利用AFM进一步研究病毒下层结构提供基础.方法 用不同浓度的非离子犁表面活性剂NP-40对完整的A型流感病毒进行处理,以轻敲模式经AFM成像,获得病毒球状体和丝状体的高度图、振幅图以及相位图,并观察和比较不同浓度非离子表面活性剂对病毒表面形态和结构的影响.结果 NP-40各浓度对病度表面破坏程度不一,病毒随NP-40浓度增高而逐渐降解,并出现部分剥离病毒表面.暴露下层衣壳,更清晰地展示包膜下层表面突起的表面形态学结果.结论 通过表面活性剂优化处理病毒颗粒,实现了利用AFM观测流感病毒包膜下形态结构的设想.

Atomic force microscopy investigation of the denudation of the envelope of influenza virus treated with Nonidet P-40

Objective Through observing the morphology and topography of the prepared influenza viruses (HIN1) treated with the different Nonidet P-40 solutions using atomic force microscopy (AFM),to explore the application of AFM on the research of the internal character of viral morphology and structural virology. Methods The virus samples were treated with serial diluted Nonidet P-40 solutions from 0.05% to 0.20% and then investigated by AFM with the tapping mode in air at room temperature to obtain the morphology and topography changes including height data,amplitude data and phase data for both spherical and filamentous influenza virus A. Results The serial AFM images show that the erosion degree of the virions is proportional with the improvement of NP-40 concentration,and partly denuded virion image appeared at 0.05 % NP-40 treatment,which was revealed clearly on both amplitude images and phase images. Conclusion This work demonstrated for the first time that the internal topography of influenza virion could be revealed by AFM via suitable nonionic surfactants chemical dissection.