| 瘦素对大鼠肝星状细胞硬脂酰辅酶A去饱和酶-1基因表达的影响 |
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| 引用本文： | 闫爽,刘思颖,封泉,崔新河. 瘦素对大鼠肝星状细胞硬脂酰辅酶A去饱和酶-1基因表达的影响[J]. 陕西医学杂志, 2010, 39(3): 295-297 |
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| 作者姓名： | 闫爽 刘思颖 封泉 崔新河 |
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| 作者单位： | 1. 哈尔滨医科大学附属第四医院内分泌科,哈尔滨,150001
2. 新疆自治区伊犁州奎屯医院 |
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| 基金项目： | 黑龙江省自然科学基金资助项目 |
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| 摘 要： | 目的:探讨瘦素(Leptin)对大鼠肝星状细胞(HSC)硬脂酰辅酶A去饱和酶-1(SCD-1)基因表达的影响,为进一步寻找糖尿病人合并脂肪肝的形成机制提供新的思路。方法:将培养好的肝星状细胞系分为两组,分别应用100ng/ml瘦素和DMEM细胞培养液培养,运用RealTimePCR的方法研究瘦素对SCD-1基因的调节作用。结果:应用RealTimePCR技术检测瘦素可以下调SCD-1基因的表达,下调倍数0.315倍。结论:高浓度瘦素可能通过下调SCD-1基因表达参与脂肪肝的形成,可能是导致糖尿病及代谢综合征合并脂肪肝的原因之一。
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| 关 键 词： | 瘦素/病理生理学 肝细胞 硬脂酰CoA去饱和酶/分析 基因表达 大鼠 |
The effect of leptin on SCD-1 gene expression of the rat hepatic satellite cell |
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| Affiliation: | Yan Shuang Liu Siying Feng Quan et al Department of Endocrinology,the Forth Affliated Hospital of Harbin Medical University(Harbin 150001) |
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| Abstract: | Objective：To study the effect of leptin on SCD-1 gene expression of the rat hepatic satellite cell.Method：Two groups（control and experiment group）were culturaled with control solution and leptin respectively.The expression of SCD-1 mRNA in the hepatic satellite cell were detected by RT-PCR.Results：The expression of SCD-1gene were down regulated in the hepatic satellite cell which culturaled with leptin,and its expression was only 0.315 of that in HSCs which culturaled with control solution.Conclusion：SCD-1 gene expression could be down-regulated by leptin,and it suggest that leptin may play an inportant role in the formation of nonalcoholic fatty liver in diabetes or metabolic syndrome by regulating the expression of SCD-1 gene. |
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| Keywords: | Leptin/physiopathology Hepatocyte Stearogl-CoA desaturase/analysis Gene expression Rats |
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