| 人胃癌细胞环氧合酶-2表达的蛋白质组双向凝胶电泳分析 |
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| 引用本文： | 高飞,潘阳林,麻树人,孙立军,梁洁,吴开春. 人胃癌细胞环氧合酶-2表达的蛋白质组双向凝胶电泳分析[J]. 世界华人消化杂志, 2005, 13(7): 840-843 |
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| 作者姓名： | 高飞 潘阳林 麻树人 孙立军 梁洁 吴开春 |
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| 作者单位： | 1. 中国人民解放军第四军医大学西京医院全军消化疾病研究所,陕西省,西安市,710033
2. 沈阳军区总医院消化内镜中心,辽宁省,沈阳市,110015 |
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| 基金项目： | 国家自然科学基金资助课题,No．39870320~~ |
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| 摘 要： |
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| 关 键 词： | 蛋白质组 胃癌 环氧合酶-2 双向凝胶电泳 |
| 修稿时间： | 2005-01-17 |
Proteomic analysis of human gastric carcinoma cells with different levels of COX-2 by two-dimensional electrophoresis |
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| Fei Gao,Yang-Lin Pan,Shu-Ren Ma,Li-Jun Sun,Jie Liang,Kai-Chun Wu. Proteomic analysis of human gastric carcinoma cells with different levels of COX-2 by two-dimensional electrophoresis[J]. World Chinese Journal of Digestology, 2005, 13(7): 840-843 |
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| Authors: | Fei Gao Yang-Lin Pan Shu-Ren Ma Li-Jun Sun Jie Liang Kai-Chun Wu |
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| Affiliation: | Fei Gao,Yang-Lin Pan,Li-Jun Sun,Jie Liang,Kai-Chun Wu,Chinese PLA Institute of Gastroenterology,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,Shanxi Province,China Shu-Ren Ma,Centerof Digestive Endoscopy,General Hosipital of Shenyang Military Command,Shenyang 110015,Liaoning Provinces China |
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| Abstract: | AIM: To establish and optimize the methods for proteome research, and to comparatively analyze the proteomes of human SGC7901-asCOX2 cells and SGC7901-pcDNA cells. METHODS: A series of methods, including immobilized pH gradient two-dimensional polyacrylamide gel electro-phoresis (2DE), silver staining, and Melanie 3 2-D analysis software, were used to analyze the difference of protein expression between SGC7901-asCOX2 cells and SGC7901-pcDNA cells. RESULTS: The 2DE pattern with good resolution and reproducibility was obtained. After silver staining, the 2DE image was analyzed by Melanie 3 2-D software. The results showed that 6 protein spots were exclusively detected in SGC7901 -pcDNA cells, and 4 spots were detected in SGC7901-asCOX2 cells. Compared to SGC7901-pcDNA cells, there were 10 protein spots downregulated in SGC7901-asCOX2, and 6 protein spots upregulated. CONCLUSION: There is a significant difference between proteomes of human gastric SGC7901-asCOX2 cells and SGC7901-pcDNA cells. |
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| Keywords: | Proteome Gastric carcinoma COX-2 Two-dimensional electrophoresis |
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