HPLC法测定四物合剂中芍药苷和阿魏酸的含量

目的:建立以高效液相色谱(HPLC)法测定四物合剂中芍药苷和阿魏酸含量的方法。方法:色谱柱为VP-ODSC18(150mm×4.6mm,5μm),流动相为乙腈-0.05%磷酸(12∶88),检测波长分别为230、323nm,柱温为40℃,流速为1mL.min-1。结果:芍药苷和阿魏酸的检测浓度分别在3.75～120.00μg.mL-1(r=0.9998)和1.13～36.25μg.mL-1(r=0.9998)范围内与各自峰面积积分值呈良好的线性关系;二者平均回收率分别为99.56%、99.89%,RSD分别为2.22%(n=9)和1.57%(n=9)。结论:本法简便、准确、重现性好,可用于四物合剂的质量控制。

Simultaneous Determination of Paeoniflorin and Ferulic Acid in Siwu Mixture by HPLC

OBJECTIVE：To establish an HPLC method to determine the contents of Paeoniflorin and Ferulic acid in Siwu mixture.METHODS：The seperation of samples was performed on a VP -ODS C18 column with column temperature maintained at 40 ℃ .The mobile phase consisted of acetonetrile-0.05% phosphoric acid（12 ： 88） at a flow rate of 1 mL · min^-1, and the detection wavelength was set at 230 nm and 323 nm, respectively.RESULTS：The calibration curves of Paeoniflorin and Ferulic acid were linear over the ranges of 3.75-120.00μg · mL^-1（r = 0.999 8） and 1.13-36.25 μg · mL^-1（r= 0.999 8） respectively.The average recoveries were 99.56% （RSD = 2.22%, n = 9） and 99.89% （RSD = 1.57%, n = 9）, respectively. CONCLUSION ： The developed method is simple, accurate and reproducible, and it can be used for quality control of Siwu mixture.