HPLC法测定栀蒡热毒平方有效部位中4种成分含量

目的 建立栀蒡热毒平方有效部位中4种成分栀子苷、京尼平-1-β-D-龙胆双糖苷、牛蒡子苷和黄芩苷的含量测定方法.方法 采用HPLC法,色谱柱为Diamonsil C_(18)柱(4.6 mm×150 mm,5μm),乙腈-0.2%磷酸水梯度洗脱,流速1.0 mL/min,检测波长238 nm和278 nm.结果 栀子苷平均回收率99.02%,RSD1.99%(n=6);京尼平-1-β-D-龙胆双糖苷平均回收率99.03%,RSD2.83%(n=6);牛蒡子苷平均回收率101.12%,RSD2.86%(n=6);黄芩苷平均回收率100.05%,RSD2.02%(n=6).结论 3批样品测定结果表明,该方法简便、准确,可用于栀蒡热毒平有效部位中栀子苷等4种成分的含量测定.

Quantitative determination of 4 chemical constituents in effective fraction f Zhibangreduping Formula by HPLC

Objective To establish a method for quantitative determination of 4 chemical constituentsgeniposide,genipin-1-β-D-gentiobioside,arctiin and baicalin in the effective fraction of Zhibangreduping Formula.Methods The procedare of HPLC was performed on the chromatographic column of Diamonsil C_(18)(4.6 mm×150 mm,5μm),and the mobile phase in the gradient elution was acetonitrile-phosphoric acid solution(0.2%).The flow rate was 1.0 mL/min and the detection wavelength was 238 nm and 278 nm.Results The average recovery of geniposide WaS 99.02%(RSD=1.99%,n=6),genipin-1-β-D-gentiobioside,99.03%(RSD=2.83%,n=6),arctiin,101.12%(RSD=2.86%,n=6),and baicalin,100.05%(RsD=2.02%.n=6).Conclusion The results of determination in 3 batches of samples show that the method is easy and accurate,which can be used in the quantitative determination of 4 chemical constituents in the effective fraction of Zhibangreduping Formula.