The effect of prefrontal stimulation on the firing of basal forebrain neurons in urethane anesthetized rat |
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| Authors: | Gyengési Erika Zaborszky Laszlo Détári László |
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| Affiliation: | 1. Eötvös Loránd University, Department of Physiology and Neurobiology, Budapest H1117, Hungary;2. Rutgers University, Center for Molecular and Behavioral Neuroscience, Newark, NJ 07102, USA;1. Department of Neurology and Clinical Neurophysiology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany;2. Laboratory for Biomechanics and Biomaterials, Department of Orthopedics, Hannover Medical School, Hannover, Germany;3. Department of Neurosurgery, Hannover Medical School, Hannover, Germany;1. Department of Psychosomatic Medicine, University of Rostock, Rostock, Germany;2. DZNE, German Center for Neurodegenerative Diseases, Rostock/Greifswald, Rostock, Germany;3. DZNE, German Center for Neurodegenerative Diseases, Rostock/Greifswald, Greifswald, Germany;4. Institute for Community Medicine, University Medicine Greifswald, Greifswald, Germany;5. Institute of Diagnostic Radiology and Neuroradiology, University Medicine Greifswald, Greifswald, Germany;6. Interfaculty Institute for Genetics and Functional Genomics, University Medicine and Ernst-Moritz-Arndt University, Greifswald, Germany;7. Department of Psychiatry and Psychotherapy, University Medicine Greifswald, Greifswald, Germany;8. Department of Psychiatry and Psychotherapy, HELIOS-Hospital Stralsund, Stralsund, Germany;1. Vision Science Graduate Group, University of California, Berkeley, Berkeley, CA 94720-2020, United States;2. Helen Wills Neuroscience Institute, and School of Optometry, University of California, Berkeley, Berkeley, CA 94720-2020, United States;1. Sorbonnes University Paris 1, MATRICE Project, ISC-PIF, 113, rue Nationale, 75013 Paris, France;2. Sorbonne University, UPMC Univ Paris 06, Laboratoire Imagerie Biomedicale, ISC-PIF, 75013 Paris, France;3. INRA-SenS, IFRIS/UPEM – Cité Descartes 5 boulevard Descartes Champs sur Marne, 77454 Marne-la-Vallée Cedex 2, France;4. University of Texas at Austin, Department of Psychology, United States;1. Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA;2. Department of Physics, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0435, USA;3. Booz Allen Hamilton, 575 Herndon Parkway, Herndon, VA 20170, USA;4. Center for Soft Matter and Biological Physics, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0435, USA;5. Academy of Integrated Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0405, USA |
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| Abstract: | The basal forebrain (BF) contains a heterogeneous population of cholinergic and non-cholinergic corticopetal neurons and interneurons. Neurons firing at a higher rate during fast cortical EEG activity (f>16Hz) were called F cells, while neurons that increase their firing rate during high-amplitude slow-cortical waves (f<4Hz) were categorized as S-cells. The prefrontal cortex (PFC) projects heavily to the BF, although little is known how it affects the firing of BF units. In this study, we investigated the effect of stimulation of the medial PFC on the firing rate of BF neurons (n=57) that were subsequently labeled by biocytin using juxtacellular filling (n=22). BF units were categorized in relation to tail-pinch induced EEG changes. Electrical stimulation of the medial PFC led to responses in 28 out of 41 F cells and in 8 out of 9 S cells. Within the sample of responsive F cells, 57% showed excitation (n=8) or excitation followed by inhibitory period (n=8). The remaining F cells expressed a short (n=6) or long inhibitory (n=6) response. In contrast, 6 out of the 8 responsive S cells reduced their firing after prefrontal stimulation. Among the F cells, we recovered one cholinergic neuron and one parvalbumin-containing (PV) neuron using juxtacellular filling and subsequent immunocytochemistry. While the PV cell displayed short latency facilitation, the cholinergic cell showed significant inhibition with much longer latency in response to the prefrontal stimulus. This is in agreement with previous anatomical data showing that prefrontal projections directly target mostly non-cholinergic cells, including GABAergic neurons. |
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| Keywords: | Cortical activation Basal forebrain Prefrontal cortex Electrical stimulation Juxtacellular labeling |
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