| 高效液相色谱法测定乳酸左氧氟沙星脂质体凝胶及霜剂中主药的含量 |
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| 引用本文： | 肖桂珍,罗顺德,张先洲,刘刚,周本宏. 高效液相色谱法测定乳酸左氧氟沙星脂质体凝胶及霜剂中主药的含量[J]. 中国药房, 2006, 17(15): 1174-1175 |
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| 作者姓名： | 肖桂珍 罗顺德 张先洲 刘刚 周本宏 |
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| 作者单位： | 1. 暨南大学珠海市人民医院,珠海市,519000
2. 武汉大学药学院,武汉市,430060 |
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| 摘 要： | 目的:建立以高效液相色谱法测定乳酸左氧氟沙星脂质体凝胶及霜剂中主药含量的方法。方法:色谱柱为ZorbaxSBC18,流动相为乙腈-0·05mol/L枸橼酸溶液-1mol/L乙酸铵溶液(30∶69·14∶0·86),流速为0·80ml/min,检测波长为293nm,进样量为20μl。结果:乳酸左氧氟沙星检测浓度在5·0~60·0μg/ml范围内线性关系良好(r=0·9998,n=6),平均回收率脂质体凝胶、霜剂分别为100·82%~101·10%(RSD=1·74%~2·88%)、100·84%~101·33%(RSD=1·81%~3·03%)。结论:该方法灵敏、稳定、重现性好,用于含量测定结果准确可靠。
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| 关 键 词： | 高效液相色谱法 乳酸左氧氟沙星 脂质体 凝胶 霜剂 含量测定 |
| 文章编号： | 1001-0408(2006)15-1174-02 |
| 收稿时间： | 2006-01-18 |
| 修稿时间： | 2006-05-10 |
Content Determination of Levofloxacin Lactate Liposomes Gel and Cream by HPLC |
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| XIAO Guizhen,LUO Shunde,ZHANG Xianzhou,LIU Gang,ZHOU Benhong. Content Determination of Levofloxacin Lactate Liposomes Gel and Cream by HPLC[J]. China Pharmacy, 2006, 17(15): 1174-1175 |
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| Authors: | XIAO Guizhen LUO Shunde ZHANG Xianzhou LIU Gang ZHOU Benhong |
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| Abstract: | OBJECTIVE:To establish a HPLC method for the content determination of levofloxacin lactate liposome gel and cream.METHODS:Zorbax C 18 column was adopted the mobile phase was acetonitrile-0.05mol/L citric acid-1mol/L ammonium acetate(30∶69.14∶0.86),the flow rate was0.80ml/min,sample size was20μl,and the detection wavelength was293nm.RESULTS:The method was linear over the range of5.0～60.0μg/ml(r=0.9998,n=6),the average recoveries of liposome gel and cream were100.82%～101.10%(RSD=1.74%～2.88%)and100.84%～101.33%(RSD=1.81%～3.03%),respectively.CONCLUSIONS:The established method is sensitive,stable,and highly reproducible,which produces an accurate and reliable result for the content determination of this preparation. |
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| Keywords: | HPLC Levofloxacin lactate Liposomes Gel Cream Content determination |
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